González-Rivera Rubén, Navarro Luz, Martínez-Vargas Marina, Guzman-Vasquez Khalil, León-Rosario Pablo, Landa Abraham, Prospero-Garcia Oscar
Depto. Fisiologia, Facultad de Medicina, UNAM. Apdo. Postal 70-250, Mexico, D.F. 04510, Mexico.
Neurosci Lett. 2006 Nov 20;408(3):178-82. doi: 10.1016/j.neulet.2006.08.087. Epub 2006 Oct 5.
It has been hypothesized that proteins modulate rapid eye movement sleep (REMS). Studies have shown an increase in the liberation of proteins in the mesencephalic reticular formation of cats during REMS. It has also been determined that protein-synthesis inhibitors diminish REMS and that protease-inhibitors increase this sleep phase. These and other studies support the importance of "di novo" protein molecules in sleep, and in particular, in REMS regulation. In this context, it is important to determine the role of endogenous proteases and their endogenous inhibitors in sleep regulation. In this study, we found that Cystatin C (CC), an endogenous protease inhibitor, diminishes wakefulness and increases REMS. We have also found an increase in CC expression after REMS deprivation and a tendency to decrease after a 2 h period of REMS rebound. We further showed that REMS deprivation increases the expression of Cathepsin H (CH), a protease inhibited by CC. These results suggest that naturally occurring protease-inhibitors enhance REMS, perhaps by facilitating the availability of proteins.
据推测，蛋白质可调节快速眼动睡眠（REMS）。研究表明，猫在快速眼动睡眠期间中脑网状结构中蛋白质的释放会增加。还已确定蛋白质合成抑制剂会减少快速眼动睡眠，而蛋白酶抑制剂会增加这个睡眠阶段。这些研究以及其他研究都支持“从头合成”蛋白质分子在睡眠中，尤其是在快速眼动睡眠调节中的重要性。在这种情况下，确定内源性蛋白酶及其内源性抑制剂在睡眠调节中的作用很重要。在本研究中，我们发现内源性蛋白酶抑制剂胱抑素C（CC）可减少清醒时间并增加快速眼动睡眠。我们还发现快速眼动睡眠剥夺后CC表达增加，而在快速眼动睡眠反弹2小时后有下降趋势。我们进一步表明，快速眼动睡眠剥夺会增加组织蛋白酶H（CH）的表达，CH是一种受CC抑制的蛋白酶。这些结果表明，天然存在的蛋白酶抑制剂可能通过促进蛋白质的可用性来增强快速眼动睡眠。
