Dakouane-Giudicelli Mbarka, Legrand Beatrice, Bergere Marianne, Giudicelli Yves, Cussenot Olivier, Selva Jacqueline
Service Histologie Embryologie Biologie de la Reproduction-Cytogénétique, Saint-Quentin, France.
Fertil Steril. 2006 Oct;86(4):873-7. doi: 10.1016/j.fertnstert.2006.03.035.
To determine whether the size of CAG repeat in exon 1 of the androgen receptor (AR) gene is related to impaired spermatogenesis in older men.
Study of two groups of older men: one with preserved spermatogenesis and the other with arrested spermatogenesis.
University teaching hospital.
PATIENT(S): Twenty-eight men aged from 53 to 102 years.
INTERVENTION(S): The DNA fragment encoding the AR polyglutamine tract was amplified from DNA of testis tissue.
MAIN OUTCOME MEASURE(S): The size of the CAG repeat was evaluated by using fluorescent-labeled polymerase chain reaction performed on an ABI Prism 377 DNA sequencer followed by automated analysis with Genscan 3.1.2 software.
RESULT(S): Mean CAG repeat length was 22.76 +/- 3 in the group of 13 aged men with preserved spermatogenesis and 21.86 +/- 2.23 in the group of 15 aged men with arrested spermatogenesis.
CONCLUSION(S): Impaired spermatogenesis in elderly men does not seem to be correlated with the AR gene CAG repeat length, which therefore does not appear to be a risk factor for impaired spermatogenesis in older men.
确定雄激素受体（AR）基因外显子1中CAG重复序列的长度是否与老年男性精子发生受损有关。
对两组老年男性进行研究：一组精子发生正常，另一组精子发生停滞。
大学教学医院。
28名年龄在53至102岁之间的男性。
从睾丸组织的DNA中扩增编码AR多聚谷氨酰胺序列的DNA片段。
使用在ABI Prism 377 DNA测序仪上进行的荧光标记聚合酶链反应，随后用Genscan 3.1.2软件进行自动分析，评估CAG重复序列的长度。
13名精子发生正常的老年男性组的平均CAG重复长度为22.76±3，15名精子发生停滞的老年男性组的平均CAG重复长度为21.86±2.23。
老年男性精子发生受损似乎与AR基因CAG重复长度无关，因此它似乎不是老年男性精子发生受损的危险因素。
