Lagacé J, Fréchette M
Department of Microbiology and Immunology, Faculty of Medicine, University of Montreal, Quebec, Canada.
Infect Immun. 1991 Feb;59(2):712-5. doi: 10.1128/iai.59.2.712-715.1991.
Monoclonal antibodies against the elastase of Pseudomonas aeruginosa were produced from spleen cells of BALB/c mice primed with purified elastase of P. aeruginosa and P3-X63-Ag8-U1 myeloma cells. The six clones established generated antibodies which reacted with a 33,000-Da peptide and recognized four different elastase epitopes by a competitive binding enzyme-linked immunosorbent assay. The monoclonal antibodies designated as ELA-17 and ELA-42 that recognize two different epitopes reacted by dot-enzyme immunoassay and by Western immunoblotting with all clinical and International Antigen Typing Scheme strains of P. aeruginosa positive for elastase.
用铜绿假单胞菌纯化弹性蛋白酶免疫的BALB/c小鼠脾细胞与P3-X63-Ag8-U1骨髓瘤细胞融合，制备出抗铜绿假单胞菌弹性蛋白酶的单克隆抗体。所建立的6个克隆产生的抗体与一个33000道尔顿的肽发生反应，通过竞争性结合酶联免疫吸附测定法识别4种不同的弹性蛋白酶表位。名为ELA-17和ELA-42的单克隆抗体识别两种不同表位，通过斑点酶免疫测定法以及与所有弹性蛋白酶呈阳性的铜绿假单胞菌临床菌株和国际抗原分型方案菌株进行蛋白质免疫印迹法反应。
