Cash H A, Straus D C, Bass J A
Can J Microbiol. 1983 Apr;29(4):448-56. doi: 10.1139/m83-072.
An experimental animal model of chronic pseudomonas pneumonia was used to document the production of potential virulence factors by Pseudomonas aeruginosa during the infection. The production of exotoxin A, proteolytic enzymes, and the serotype-specific lipopolysaccharide and slime-layer antigens during the infection was examined by solid-phase radioimmunoassay of serum from infected rats and by indirect immunofluorescence tests of their lung tissue. Rats inoculated intratracheally with purified bacterial exoproducts, delivered alone or in combination, developed pulmonary histopathology similar to that induced by the experimental infection. The results indicate that these exoproducts are produced during the course of the pulmonary infection and suggest that they are involved in the observed lung pathology.
使用慢性铜绿假单胞菌肺炎实验动物模型来记录铜绿假单胞菌在感染过程中潜在毒力因子的产生。通过对感染大鼠血清进行固相放射免疫测定以及对其肺组织进行间接免疫荧光试验，检测感染期间外毒素A、蛋白水解酶以及血清型特异性脂多糖和黏液层抗原的产生情况。经气管内接种单独或联合递送的纯化细菌外产物的大鼠，出现了与实验性感染诱导的肺部组织病理学相似的情况。结果表明，这些外产物在肺部感染过程中产生，并提示它们与观察到的肺部病理学有关。
