Bradham Cynthia A, Foltz Kathy R, Beane Wendy S, Arnone Maria I, Rizzo Francesca, Coffman James A, Mushegian Arcady, Goel Manisha, Morales Julia, Geneviere Anne-Marie, Lapraz François, Robertson Anthony J, Kelkar Hemant, Loza-Coll Mariano, Townley Ian K, Raisch Michael, Roux Michelle M, Lepage Thierry, Gache Christian, McClay David R, Manning Gerard
DCMB Group, Duke University, Box 91000, Durham, NC 27708, USA.
Dev Biol. 2006 Dec 1;300(1):180-93. doi: 10.1016/j.ydbio.2006.08.074. Epub 2006 Sep 12.
This paper reports a preliminary in silico analysis of the sea urchin kinome. The predicted protein kinases in the sea urchin genome were identified, annotated and classified, according to both function and kinase domain taxonomy. The results show that the sea urchin kinome, consisting of 353 protein kinases, is closer to the Drosophila kinome (239) than the human kinome (518) with respect to total kinase number. However, the diversity of sea urchin kinases is surprisingly similar to humans, since the urchin kinome is missing only 4 of 186 human subfamilies, while Drosophila lacks 24. Thus, the sea urchin kinome combines the simplicity of a non-duplicated genome with the diversity of function and signaling previously considered to be vertebrate-specific. More than half of the sea urchin kinases are involved with signal transduction, and approximately 88% of the signaling kinases are expressed in the developing embryo. These results support the strength of this nonchordate deuterostome as a pivotal developmental and evolutionary model organism.
本文报道了对海胆激酶组的初步计算机分析。根据功能和激酶结构域分类法，对海胆基因组中预测的蛋白激酶进行了鉴定、注释和分类。结果表明，就激酶总数而言，由353种蛋白激酶组成的海胆激酶组比人类激酶组（518种）更接近果蝇激酶组（239种）。然而，海胆激酶的多样性与人类惊人地相似，因为海胆激酶组在186个人类亚家族中仅缺失4个，而果蝇则缺失24个。因此，海胆激酶组将无重复基因组的简单性与先前认为是脊椎动物特有的功能和信号传导多样性结合在一起。超过一半的海胆激酶参与信号转导，并且大约88%的信号激酶在发育中的胚胎中表达。这些结果支持了这种非脊索动物后口动物作为关键的发育和进化模式生物的优势。
