Stazione Zoologica Anton Dohrn, Department of Ecosustainable Marine Biotechnology, Via Ammiraglio Ferdinando Acton n. 55, 80133 Napoli, Italy.
Stazione Zoologica Anton Dohrn, Department of Ecosustainable Marine Biotechnology, Calabria Marine Centre, C.da Torre Spaccata, 87071 Amendolara, Italy.
Int J Mol Sci. 2024 Jan 30;25(3):1685. doi: 10.3390/ijms25031685.
The Mediterranean purple sea urchin (Lamarck 1816) is a remarkable model system for molecular, evolutionary and cell biology studies, particularly in the field of developmental biology. We sequenced the genome, performed a assembly, and analysed the assembly content. The genome of was sequenced using Illumina NextSeq 500 System (Illumina) in a 2 × 150 paired-end format. More than 30,000 open reading frames (ORFs), (more than 8000 are unique), were identified and analysed to provide molecular tools accessible for the scientific community. In particular, several genes involved in complex innate immune responses, oxidative metabolism, signal transduction, and kinome, as well as genes regulating the membrane receptors, were identified in the genome. In this way, the employment of the Mediterranean sea urchin for investigations and comparative analyses was empowered, leading to the explanation of cis-regulatory networks and their evolution in a key developmental model occupying an important evolutionary position with respect to vertebrates and humans.
地中海紫海胆（Lamarck 1816）是分子、进化和细胞生物学研究，特别是在发育生物学领域的一个重要模型系统。我们对其基因组进行了测序、组装，并对组装内容进行了分析。我们使用 Illumina NextSeq 500 系统（Illumina）对 的基因组进行了测序，采用 2×150 配对末端格式。我们鉴定并分析了超过 30000 个开放阅读框（ORFs）（其中超过 8000 个是独特的），为科学界提供了易于获取的分子工具。特别是，在 基因组中鉴定出了几个参与复杂先天免疫反应、氧化代谢、信号转导和激酶组以及调节膜受体的基因。通过这种方式，我们可以利用地中海海胆进行研究和比较分析，从而解释顺式调控网络及其在一个相对于脊椎动物和人类具有重要进化地位的关键发育模型中的进化。
