Collen T, Dimarchi R, Doel T R
Vaccine Research Department, AFRC Institute for Animal Health, Pirbright, Surrey, England.
J Immunol. 1991 Jan 15;146(2):749-55.
Synthetic peptides representing regions of the VP1 protein of foot-and-mouth disease virus strain 01 Kaufbeuren were screened for their ability to stimulate proliferation of PBMC from virus vaccinated cattle. Sites were identified at residue 21-40 (peptide FMDV32) and in the region C-terminal to residue 161. Cells responding to FMDV32 were MHC class II-restricted, CD4+ and secreted IL-2. Thus, this region is defined as a Th site. Of 19 virus vaccinated Friesian cattle, 89% (17/19) responded to purified virus while 37% (7/19; 41% of virus responders) also responded to FMDV32 suggesting that this site is immunodominant for the cattle used. Furthermore, immunisation of FMDV32 responder and non-responder cattle with a related peptide, FMDV5 (FMDV32 co-linearly synthesized with the 141-160 VP1 B cell site), induced neutralizing antibody and a virus-specific T cell population in the FMDV32-responder but not the non-responder animals.
对代表口蹄疫病毒01考夫博伊伦株VP1蛋白区域的合成肽进行了筛选，以检测其刺激接种病毒的牛外周血单核细胞（PBMC）增殖的能力。在第21 - 40位氨基酸残基处（肽FMDV32）以及第161位氨基酸残基C末端区域发现了相关位点。对FMDV32有反应的细胞受MHC II类分子限制，为CD4⁺ 细胞并分泌白细胞介素-2。因此，该区域被定义为一个Th细胞表位。在19头接种病毒的弗里斯兰牛中，89%（17/19）对纯化病毒有反应，而37%（7/19；占病毒反应者的41%）也对FMDV32有反应，这表明该位点对所使用的牛具有免疫显性。此外，用相关肽FMDV5（与141 - 160位VP1 B细胞表位共线性合成的FMDV32）免疫FMDV32反应者和非反应者牛，在FMDV32反应者动物中诱导产生了中和抗体和病毒特异性T细胞群体，而在非反应者动物中则未诱导产生。
