Novikov D K, Dotsenko E A, Zhakova G Ia
Lab Delo. 1990(9):58-61.
Basophil degranulation test (BDT) was used in examinations of patients suffering from allergies. A modified technique was employed with toluidine blue staining and basophil scintillation in a counter chamber. The examinees were divided into three groups. Group 1 (n = 34) consisted of patients suffering from atopic bronchial asthma with suspected sensitization to home dust. Group 2 included 118 patients with a history of allergy to penicillin. Group 3 consisted of 51 patients with drug allergies (streptomycin, vitamin B1, procaine, analgin intolerance). In all the cases specific diagnosis was confirmed by skin and provocation test. 71.4 percent of the patients with sensitization to home dust allergen showed positive results in BDT, as did 68.6 percent of Group 3 subjects. Of the cases with penicillin sensitization confirmed by application test 91.7 percent were positive in BDT, of those with positive intradermal tests, 66.7 percent were BDT-positive, this being probably explained by individual sensitivity of the patients. These results recommend BDT, an informative and simple technique, for wide practice.
嗜碱性粒细胞脱粒试验（BDT）用于过敏患者的检查。采用改良技术，在计数室中用甲苯胺蓝染色和嗜碱性粒细胞闪烁法。受试者分为三组。第一组（n = 34）由疑似对家庭灰尘过敏的特应性支气管哮喘患者组成。第二组包括118例有青霉素过敏史的患者。第三组由51例药物过敏（链霉素、维生素B1、普鲁卡因、安乃近不耐受）患者组成。在所有病例中，特异性诊断均通过皮肤试验和激发试验得以证实。对家庭灰尘过敏原敏感的患者中，71.4%的BDT结果呈阳性，第三组受试者中这一比例为68.6%。在应用试验证实有青霉素致敏的病例中，91.7%的BDT结果呈阳性，在皮内试验呈阳性的病例中，66.7%的BDT结果呈阳性，这可能是由患者的个体敏感性所致。这些结果表明BDT是一种信息丰富且简单的技术，值得广泛应用。
