Chen Zuyu, Zhang Jingjing, Kong Jin, Li Shaoqing, Fu Yan, Li Shaobo, Zhang Hong, Li Yangsheng, Zhu Yingguo
Key Laboratory of MOE for Plant Developmental Biology, College of Life Science, Wuhan University, 430072, Wuhan, P R China.
Genetica. 2006 Sep-Nov;128(1-3):21-31. doi: 10.1007/s10709-005-2486-0.
Small non-coding RNAs play important roles in regulating cell functions by controlling mRNA turnover and translational repression in eukaryotic cells. Here we isolated 162 endogenous small RNA molecules from Oryza sativa, which ranged from 16 to 35 nt in length. Further analysis indicated that they represented a diversity of small RNA molecules, including 17 microRNAs (miRNAs), 30 tiny non-coding RNAs (tncRNAs) and 20 repeat-associated small interfering RNAs (rasiRNAs). Among 17 miRNAs, 13 were novel miRNA candidates and their potential targets were important regulatory genes in the rice genome. We also found that a cluster of small RNAs, including many rasiRNAs, matched to a nuclear DNA fragment that evolutionarily derived from chloroplast. These results demonstrate clearly the existence of distinct types of small RNAs in rice and further suggest that small RNAs may control gene regulation through diverse mechanisms.
小非编码RNA通过控制真核细胞中的mRNA周转和翻译抑制在调节细胞功能中发挥重要作用。在此，我们从水稻中分离出162个内源性小RNA分子，其长度在16至35个核苷酸之间。进一步分析表明，它们代表了多种小RNA分子，包括17个微小RNA（miRNA）、30个微小非编码RNA（tncRNA）和20个重复相关小干扰RNA（rasiRNA）。在17个miRNA中，有13个是新的miRNA候选物，它们的潜在靶标是水稻基因组中的重要调控基因。我们还发现，一组小RNA，包括许多rasiRNA，与一个进化上源自叶绿体的核DNA片段相匹配。这些结果清楚地证明了水稻中存在不同类型的小RNA，并进一步表明小RNA可能通过多种机制控制基因调控。
