Schultz Edward, Chamberlain Connie, McCormick Kathleen M, Mozdziak Paul E
Department of Anatomy, University of Wisconsin, Madison Wisconsin, USA.
Dev Dyn. 2006 Dec;235(12):3230-9. doi: 10.1002/dvdy.20976.
Satellite cells are the myogenic cells lying between the myofiber sarcolemma and basal lamina. The objective of this study was to determine the expression patterns of MyoD, myogenin, and Pax7 within the satellite cell population in the growing rat soleus and extensor digitorum longus (EDL) muscles. Secondly, the expression of the myogenic markers was also studied within the interstitial cell compartment and myonuclei. It was discovered that the soleus contained a higher number of Pax7, MyoD, or myogenin-positive nuclei compared with the EDL. Similarly, myogenin was expressed at a lower level in the myonuclei of the soleus compared with the EDL, and myogenin was expressed at a higher level in the interstitial compartment of the soleus compared with the EDL. When interstitial nuclei, myonuclei, and double-labeled nuclei were used in the estimate of the satellite cell population, it was discovered that approximately of 13% of the myofibers in a transverse section of the soleus muscle and 4.1% of EDL myofibers exhibit a labeled satellite cell nucleus. Overall, results from this study suggest that expression patterns of these markers vary predictably among muscles with different growth dynamics and phenotypic characteristics.
卫星细胞是位于肌纤维肌膜和基膜之间的生肌细胞。本研究的目的是确定生长中的大鼠比目鱼肌和趾长伸肌（EDL）肌肉卫星细胞群体中MyoD、肌细胞生成素和Pax7的表达模式。其次，还研究了生肌标记物在间质细胞区室和肌核中的表达。结果发现，与EDL相比，比目鱼肌中Pax7、MyoD或肌细胞生成素阳性核的数量更多。同样，与EDL相比，比目鱼肌肌核中肌细胞生成素的表达水平较低，而与EDL相比，比目鱼肌间质区室中肌细胞生成素的表达水平较高。当使用间质核、肌核和双标记核来估计卫星细胞群体时，发现比目鱼肌横切面上约13%的肌纤维和EDL肌纤维的4.1%呈现标记的卫星细胞核。总体而言，本研究结果表明，这些标记物的表达模式在具有不同生长动力学和表型特征的肌肉之间可预测地变化。
