Salomi B S B, Mitra Chanchal K
School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
Biosens Bioelectron. 2007 Mar 15;22(8):1825-9. doi: 10.1016/j.bios.2006.08.042. Epub 2006 Oct 6.
The present study aims at investigating the use of redox dyes as non-diffusional electron mediators in hydrogen peroxide biosensors using horseradish peroxidase (HRP). We observe that the two redox dyes Safranine O and Neutral Red covalently bound to HRP, efficiently mediate electron transfer from the active site of the enzyme to the electrode surface. Dyes bound to the enzyme using a spacer arm diaminohexane further enhance the electron transfer. The enzyme electrodes show a linear response to the concentration of H2O2 up to 500 microM concentration and with a detection limit of around 50 microM. The dyes can be used as coupled mediators to develop a successful electro-optical biosensor.
本研究旨在探讨氧化还原染料作为非扩散性电子媒介体在使用辣根过氧化物酶（HRP）的过氧化氢生物传感器中的应用。我们观察到，与HRP共价结合的两种氧化还原染料，番红O和中性红，能有效地介导电子从酶的活性位点转移到电极表面。使用间隔臂二氨基己烷与酶结合的染料进一步增强了电子转移。酶电极对高达500微摩尔浓度的H2O2浓度呈现线性响应，检测限约为50微摩尔。这些染料可用作偶联媒介体来开发一种成功的电光生物传感器。
