Guijarro Patricia, Simó Sergi, Pascual Marta, Abasolo Ibane, Del Río José A, Soriano Eduardo
Institute for Research in Biomedicine (IRB)-Barcelona Science Park (PCB) and Department of Cell Biology, University of Barcelona, Josep Samitier 1-5, E-08028 Barcelona, Spain.
Mol Cell Neurosci. 2006 Dec;33(4):389-400. doi: 10.1016/j.mcn.2006.08.010. Epub 2006 Oct 9.
Few studies have addressed the issue of how GABAergic interneurons in the cerebellar cortex migrate or what guidance cues steer them. Recent data show that their development starts at the cerebellar germinal epithelium on top of the fourth ventricle. These interneurons continue to proliferate in the postnatal cerebellar white matter and later migrate to their final position in the cerebellar cortex. Here we report the chemorepulsive action of Netrin1 on postnatal cerebellar interneurons in vitro and also show the expression pattern of Netrin1 and its receptors Dcc and Unc5. Our expression results further suggest that Netrin1 is involved in the migration of GABAergic interneurons in vivo. Moreover, our data point to Bergmann glial fibers as possible tracks for these cells en route to the molecular layer. Finally, experiments using blocking antibodies allow us to conclude that Dcc, although expressed by postnatal cerebellar interneurons, is not involved in the repulsive response triggered by Netrin1 in these cells.
很少有研究探讨小脑皮质中的γ-氨基丁酸能中间神经元如何迁移，或者是什么引导线索指引它们迁移。最近的数据表明，它们的发育始于第四脑室顶部的小脑生发上皮。这些中间神经元在出生后的小脑白质中持续增殖，随后迁移到小脑皮质中的最终位置。在这里，我们报告了Netrin1对出生后小脑中间神经元的体外化学排斥作用，并且还展示了Netrin1及其受体Dcc和Unc5的表达模式。我们的表达结果进一步表明，Netrin1参与了体内γ-氨基丁酸能中间神经元的迁移。此外，我们的数据表明伯格曼胶质纤维可能是这些细胞通向分子层途中的轨迹。最后，使用阻断抗体的实验使我们得出结论，尽管出生后的小脑中间神经元表达Dcc，但它不参与Netrin1在这些细胞中引发的排斥反应。
