Williams L Keoki, McPhee Roderick A, Ownby Dennis R, Peterson Edward L, James Melissa, Zoratti Edward M, Johnson Christine C
Department of Internal Medicine, Henry Ford Health System, Detroit, MI 48202, USA.
J Allergy Clin Immunol. 2006 Oct;118(4):851-7. doi: 10.1016/j.jaci.2006.07.007. Epub 2006 Aug 30.
Both endotoxin exposure and a single nucleotide polymorphism in one of its receptors, CD14 C-260T, have been separately associated with total serum IgE levels. Furred pets might also influence IgE levels through their effects on endotoxin levels. However, how these factors interact to influence total IgE levels is not well known, especially in adults.
We sought to investigate the interactive relationship between endotoxin levels, pet exposure, and CD14 C-260T genotype on total serum IgE levels in adults.
Mothers enrolled in an ongoing cohort study were genotyped for the CD14 C-260T polymorphism. Exposure to pets was assessed by using questionnaires and dust allergen levels collected in the home. Endotoxin exposure was estimated by using dust collected from mothers' bedroom floors. The primary outcome measure was total serum IgE level.
CD14 C-260T genotype was assessed in 517 (85.2%) of the 607 women enrolled in the study. The CD14 C-260T genotype was significantly associated with total IgE levels; however, this relationship appeared to be modified by the level of endotoxin exposure. Similar interactions between CD14 C-260T and pet exposure were not seen, regardless of the measure of pet exposure used.
The CD14 C-260T genotype and endotoxin exposure together appear to influence total serum IgE levels in adults. The absence of a similar gene-environment interaction for pet exposure suggests separate mechanisms of action.
A common polymorphism in the endotoxin receptor, CD14 C-260T, and dust endotoxin levels in the home might interact to influence total serum IgE levels into adulthood.
内毒素暴露及其一种受体CD14 C-260T中的单核苷酸多态性均已分别与血清总IgE水平相关。有毛宠物也可能通过对内毒素水平的影响来影响IgE水平。然而，这些因素如何相互作用以影响总IgE水平尚不清楚，尤其是在成年人中。
我们试图研究内毒素水平、宠物接触情况和CD14 C-260T基因型对成年人血清总IgE水平的交互关系。
对参与一项正在进行的队列研究的母亲进行CD14 C-260T多态性基因分型。通过问卷调查和家中收集的灰尘过敏原水平评估宠物接触情况。通过使用从母亲卧室地板收集的灰尘来估计内毒素暴露情况。主要结局指标为血清总IgE水平。
在该研究纳入的607名女性中，对517名（85.2%）进行了CD14 C-260T基因型评估。CD14 C-260T基因型与总IgE水平显著相关；然而，这种关系似乎受到内毒素暴露水平的影响。无论使用何种宠物接触测量方法，均未观察到CD14 C-260T与宠物接触之间存在类似的相互作用。
CD14 C-260T基因型和内毒素暴露共同似乎影响成年人的血清总IgE水平。宠物接触不存在类似的基因-环境相互作用，提示其作用机制不同。
内毒素受体中的常见多态性CD14 C-260T和家中灰尘内毒素水平可能相互作用，影响成年后的血清总IgE水平。
