Schneider W, Siems W, Grune T
Institute of Biochemistry, Medical Faculty Charité, Berlin, G.D.R.
Cell Biochem Funct. 1990 Oct;8(4):227-32. doi: 10.1002/cbf.290080407.
A method for the quantification of energy consuming processes described by Siems et al. for reticulocytes and by Müller et al. for ascites tumour cells was applied to balance the ATP-consumption of isolated rat hepatocytes. On the basis of decreased coupled respiration rates following the specific inhibition of energy-requiring reactions, the energy demands of protein turnover, nucleic acid synthesis, Na+/K(+)-ATPase and Ca2(+)-transport of hepatocytes in different incubation media were assessed. These processes together with urea synthesis account for about 60 per cent of the total energy consumption in a glucose and amino acid-enriched Eagle/Borsook medium. The metabolic flux rates of total ATP-consumption and ATP-consumption of single energy-requiring processes in hepatocytes are compared with those in reticulocytes and different tumour cell types.
将Siems等人描述的用于网织红细胞以及Müller等人描述的用于腹水肿瘤细胞的能量消耗过程定量方法，应用于平衡分离的大鼠肝细胞的ATP消耗。基于在特异性抑制需能反应后耦合呼吸速率的降低，评估了不同孵育培养基中肝细胞蛋白质周转、核酸合成、Na+/K(+)-ATP酶和Ca2(+)转运的能量需求。在富含葡萄糖和氨基酸的伊格尔/博尔索克培养基中，这些过程与尿素合成一起约占总能量消耗的60%。将肝细胞中总ATP消耗和单个需能过程的ATP消耗的代谢通量率与网织红细胞和不同肿瘤细胞类型中的通量率进行比较。
