在富含氨基酸的培养基中，兔网织红细胞的ATP生成和消耗增加。
ATP production and consumption of rabbit reticulocytes increase in an amino-acid-enriched medium.
作者信息
Siems W, Müller M, Dubiel W, Dumdey R, Rapoport S
出版信息
Biomed Biochim Acta. 1986;45(5):585-91.
The ATP production and the main processes of ATP consumption, globin synthesis, Na+, K+-ATPase, and proteolysis of rabbit reticulocytes in two different media (1. complete medium with amino acids and glucose and 2. glucose containing electrolyte medium) were measured. The ATP formation in the complete medium amounts to 157 mmol/l X h which is 28% higher than in the incomplete medium. The increased ATP formation rate is due to increased ATP consumption by the more than doubled protein synthesis in the complete medium, whereas proteolysis and Na+,K+-ATPase are nearly unchanged. The increase in the glucose consumption in the complete medium is not fully accounted for by the increased oxidative degradation of glucose in the citric acid cycle and by lactate accumulation.
测定了兔网织红细胞在两种不同培养基（1. 含有氨基酸和葡萄糖的完全培养基；2. 含葡萄糖的电解质培养基）中的ATP生成以及ATP消耗的主要过程，包括珠蛋白合成、Na⁺，K⁺-ATP酶和蛋白水解。完全培养基中的ATP生成量为157 mmol/l×h，比不完全培养基高28%。ATP生成速率的增加是由于完全培养基中蛋白质合成增加了一倍多，从而导致ATP消耗增加，而蛋白水解和Na⁺，K⁺-ATP酶几乎没有变化。完全培养基中葡萄糖消耗的增加不能完全由柠檬酸循环中葡萄糖氧化降解的增加和乳酸积累来解释。
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