Orth Dorothea, Grif Katharina, Dierich Manfred P, Würzner Reinhard
Department of Hygiene, Microbiology and Social Medicine, Innsbruck Medical University and Austrian Reference Laboratory for Enterohaemorrhagic Escherichia coli, Schöpfstr. 41, A-6020 Innsbruck, Austria.
J Med Microbiol. 2006 Nov;55(Pt 11):1487-1492. doi: 10.1099/jmm.0.46666-0.
To assess the prevalence of cytolethal distending toxin (CDT) among Shiga toxin-producing Escherichia coli (STEC), 202 STEC strains were investigated using PCRs targeting various cdt alleles (cdt-I to cdt-V). Seven of the 202 strains contained cdt-III and an additional seven contained cdt-V. All 14 cdt-positive strains produced biologically active CDT, as demonstrated by a progressive distension of cultured Chinese hamster ovary cells. The CDT-positive STEC belonged to eight different serotypes, including sorbitol-fermenting O157 : NM (non-motile). The data demonstrate that CDT is present in some STEC serotypes only. However, more studies are required to evaluate whether CDT presence is associated with severe disease.
为评估产志贺毒素大肠杆菌（STEC）中细胞致死膨胀毒素（CDT）的流行情况，使用针对各种cdt等位基因（cdt-I至cdt-V）的聚合酶链反应（PCR）对202株STEC菌株进行了研究。202株菌株中有7株含有cdt-III，另有7株含有cdt-V。所有14株cdt阳性菌株均产生具有生物活性的CDT，培养的中国仓鼠卵巢细胞逐渐膨胀证明了这一点。CDT阳性的STEC属于8种不同血清型，包括发酵山梨醇的O157：NM（无动力）。数据表明，CDT仅存在于某些STEC血清型中。然而，需要更多研究来评估CDT的存在是否与严重疾病有关。
