Scarano Wellerson Rodrigo, Vilamaior Patricia Simone Leite, Taboga Sebastião Roberto
Cell Biology Department, Biology Institute, UNICAMP, Campinas, São Paulo, Brazil.
Anat Rec A Discov Mol Cell Evol Biol. 2006 Nov;288(11):1190-200. doi: 10.1002/ar.a.20391.
Prostate differentiation during embryogenesis and its further homeostatic state maintenance during adult life depend on androgens. Abundant biological data suggest that androgens play an important role in the development of the prostate cancer and other prostatic diseases. The objective of this work was to evaluate the effects of the testosterone supplementation in gerbil (a new experimental model) at different ages. Tissues from experimental animals were studied by histological and histochemistry procedures, androgen receptor immunohistochemistry assay, morphometric-stereological analysis, and transmission electron microscopy (TEM). After the treatment were observed increase of prostate weight and epithelium height in all ages studied. In some adult and aged treated animals, hyperplasic and dysplastic process were observed, including prostatic intraepithelial neoplasias and adenocarcinomas. Increase of the thickness of the smooth muscle cell (SMC) layer was observed in pubescent and adult animals and TEM revealed apparent SMC hypertrophy. An apparent increase in the frequency of blood vessels distributed by the subepithelial stroma in the treated animals was noticed. Reversion of the natural effects of aging on the prostate was observed in the aged treated animals in some acini of the gland. These data demonstrate that the gerbil prostate is susceptible to androgenic action at the studied ages and it can serve, for example, as experimental model to studies of prostate neoplastic process induction and hormonal therapy in aged animals.
胚胎发育过程中的前列腺分化及其成年期进一步的稳态维持依赖于雄激素。大量生物学数据表明，雄激素在前列腺癌及其他前列腺疾病的发展中起重要作用。本研究的目的是评估在不同年龄的沙鼠（一种新的实验模型）中补充睾酮的效果。通过组织学和组织化学程序、雄激素受体免疫组织化学分析、形态计量-体视学分析以及透射电子显微镜（TEM）对实验动物的组织进行研究。治疗后，在所有研究年龄组中均观察到前列腺重量增加和上皮高度增加。在一些成年和老年治疗动物中，观察到增生和发育异常过程，包括前列腺上皮内瘤变和腺癌。在青春期和成年动物中观察到平滑肌细胞（SMC）层厚度增加，TEM显示明显的SMC肥大。注意到治疗动物的上皮下基质中分布的血管频率明显增加。在老年治疗动物的腺体一些腺泡中观察到衰老对前列腺的自然影响的逆转。这些数据表明，在所研究的年龄，沙鼠前列腺对雄激素作用敏感，例如，它可作为研究老年动物前列腺肿瘤发生过程诱导和激素治疗的实验模型。
