Lien Tu-Fa, Chen Wenlung, Hsu Yu-Lin, Chen Hsiu-Ling, Chiou Robin Y-Y
Department of Animal Science, National Chiayi University, Chiayi, Taiwan.
J Agric Food Chem. 2006 Oct 18;54(21):8027-32. doi: 10.1021/jf061108m.
Health benefits of soy isoflavones have attracted the concern of the public and the interest of health-care professionals. In this study, two trials were conducted in characterizing bone-related traits and lens proteins as affected by supplementation of soy aglycon isoflavones (SAI). In trial 1, an in vivo study, 20 Sprague-Dawley rats were ovariectomized (OVX) and randomly distributed into OVX and OVX+SAI (135 mg of SAI/kg of feed; 8.33 mg/kg body weight; 2.5 mg/day) groups. Another group containing 10 rats with a sham operation was control (Sham). The experiment period was 3 months, and the rats were subjected to bone-related traits and lens protein characterization. In trial 2, an in vitro study, osteoprogenitor cells (UMR-106) were divided into SAI-supplemented (0.5 mg of SAI/mL of medium) and unsupplemented groups. Results of the in vivo study indicated that daily BW gains in the OVX and OVX+SAI groups were greater than that of the Sham group (p < 0.05). Bone ash and Ca contents of the Sham and OVX+SAI groups were higher than those of the OVX group (p < 0.05), while bone density, strength, and phosphorus contents among groups varied insignificantly (p > 0.05). When the lens proteins were extracted and analyzed with size-exclusion HPLC, the contents of beta- and gamma-crystallins were lowest in the OVX group and the protein solubility decrease could be recovered by dietary SAI supplementation (shown by OVX+SAI group). Based on Raman spectra of the isolated lens proteins, disulfide bonds were observed more in OVX lens than in the Sham and OVX+SAI lens. Results of in vitro study with osteoprogenitor cells revealed that cell viability, alkaline phosphatase activity, osteocalcin, and Ca contents of the SAI-supplemented group were higher than those of the unsupplemented group (p < 0.05). The likely potency to enhance bone and lens health by SAI supplementation is worth pointing out.
大豆异黄酮对健康的益处已引起公众的关注和医疗保健专业人员的兴趣。在本研究中，进行了两项试验，以表征补充大豆苷元异黄酮（SAI）对骨骼相关性状和晶状体蛋白的影响。在试验1中，一项体内研究，将20只Sprague-Dawley大鼠进行卵巢切除（OVX），并随机分为OVX组和OVX + SAI组（135 mg SAI/kg饲料；8.33 mg/kg体重；2.5 mg/天）。另一组包含10只接受假手术的大鼠作为对照（假手术组）。实验期为3个月，对大鼠进行骨骼相关性状和晶状体蛋白表征。在试验2中，一项体外研究，将成骨祖细胞（UMR-106）分为补充SAI组（0.5 mg SAI/mL培养基）和未补充组。体内研究结果表明，OVX组和OVX + SAI组的每日体重增加大于假手术组（p < 0.05）。假手术组和OVX + SAI组的骨灰和钙含量高于OVX组（p < 0.05），而各组之间的骨密度、强度和磷含量差异不显著（p > 0.05）。当用尺寸排阻HPLC提取和分析晶状体蛋白时，β-和γ-晶状体蛋白的含量在OVX组中最低，并且通过饮食补充SAI可以恢复蛋白质溶解度降低的情况（如OVX + SAI组所示）。基于分离的晶状体蛋白的拉曼光谱，在OVX晶状体中比在假手术组和OVX + SAI晶状体中观察到更多的二硫键。成骨祖细胞的体外研究结果表明，补充SAI组的细胞活力、碱性磷酸酶活性、骨钙素和钙含量高于未补充组（p < 0.05）。值得指出的是，补充SAI可能具有增强骨骼和晶状体健康的潜力。
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