大豆异黄酮与补充钙可预防大鼠卵巢切除术后骨量丢失并影响胰岛素样生长因子（IGF）-I
[Soy isoflavones with supplemental calcium provide protection against the loss of bone mass and influence insulin-like growth factor (IGF)-I after ovariectomy in rat].
作者信息
Cheng Mao-wei, Liu Jia-fa, Yi Guo-qin, Zhang Xi-ping, Zhu Qing-hua, Liu Lie-gang
机构信息
Inspcetion Part of Hygiece Inspcetion and Guard Institute of Control and Prevention of Hubei Provincial Center for Disease Control and Prevention, Wuhan 430079, China.
出版信息
Zhonghua Yu Fang Yi Xue Za Zhi. 2006 Sep;40(5):328-31.
OBJECTIVE
To compare the effects of soy isoflavone with supplemental calcium to soy isoflavone or Ca alone on preservation of bone mineral density (BMD) and the expression of insulin-like growth factor (IGF)-I.
METHODS
Sprague-Dawley (SD) female Rats, 6 months old, were ovariectomized and randomized into five groups: sham-operated group (n = 10) or ovx (n = 40) group. Shams were fed a 3.272 g/kg Ca diet. Ovx rats were randomized to a 3.272 g/kg Ca diet alone (OVX) or with soy isoflavone (SI) extract (37.95 mg/kg.bw) or to a supplemental Ca diet (Ca, 4.676 g/kg) alone or a supplemental Ca diet with the isoflavone extract (SI + Ca) for 12 weeks. BMD of femur was measured by scanner of bone mineral density. The level of IGF-1 mRNA expression was measured by reverse transcriptase-polymerase chain reaction (RT-PCR), respectively.
RESULTS
There was no significant difference between group Sham (0.267 +/- 0.008) and group SI + Ca (0.263 +/- 0.007) g/cm(2) (P > 0.05) on femur BMD of distal end. Femur BMD of distal end in group Sham and group SI + Ca was greater (P < 0.05) as compared to group OVX (0.245 +/- 0.005) g/cm(2), SI (0.258 +/- 0.011) g/cm(2) or Ca (0.255 +/- 0.004) g/cm(2), P < 0.05. The liver tissue IGF-1 mRNA contents (IGF-1 cDNA/B-actin cDNA) were significantly decreased in group Sham (0.200 +/- 0.023) g/cm(2), SI (0.278 +/- 0.019) g/cm(2), Ca (0.302 +/- 0.026) g/cm(2) or SI + Ca (0.231 +/- 0.025) g/cm(2) as compared to group OVX (0.362 +/- 0.031) g/cm(2), P < 0.05; The liver tissue IGF-1 mRNA contents (IGF-1 cDNA/B-actin cDNA) were significantly decreased in group SI + Ca (0.231 +/- 0.025) g/cm(2) compared to group SI (0.278 +/- 0.019) g/cm(2) and Ca (0.302 +/- 0.026) g/cm(2), P < 0.05.
CONCLUSIONS
Soy isoflavones combined with supplemental Ca are more protective against the loss of femur BMD than soy isoflavones or supplemental Ca diet alone. The dose of SI (37.95 mg/kg.bw) might significantly restrain the rising of the liver tissue IGF-1 mRNA contents caused by ovariectomy.
目的
比较大豆异黄酮联合补充钙与单独使用大豆异黄酮或钙对骨矿物质密度（BMD）保存及胰岛素样生长因子（IGF）-I表达的影响。
方法
将6月龄的Sprague-Dawley（SD）雌性大鼠进行卵巢切除，并随机分为五组：假手术组（n = 10）或去卵巢（ovx，n = 40）组。假手术组大鼠喂食钙含量为3.272 g/kg的饲料。去卵巢大鼠随机分为单独喂食钙含量为3.272 g/kg的饲料（OVX）或添加大豆异黄酮（SI）提取物（37.95 mg/kg体重）的饲料，或单独喂食补充钙的饲料（Ca，4.676 g/kg）或添加异黄酮提取物的补充钙饲料（SI + Ca），持续12周。用骨密度扫描仪测量股骨的骨密度。分别用逆转录聚合酶链反应（RT-PCR）测量IGF-1 mRNA表达水平。
结果
假手术组（0.267±0.008）与SI + Ca组（0.263±0.007）g/cm²在股骨远端骨密度上无显著差异（P>0.05）。假手术组和SI + Ca组股骨远端骨密度高于OVX组（0.245±0.005）g/cm²、SI组（0.258±0.011）g/cm²或Ca组（0.255±0.004）g/cm²，差异有统计学意义（P<0.05）。与OVX组（0.362±0.031）g/cm²相比，假手术组（0.200±0.023）g/cm²、SI组（0.278±0.019）g/cm²、Ca组（0.302±0.026）g/cm²或SI + Ca组（0.231±0.025）g/cm²的肝脏组织IGF-1 mRNA含量（IGF-1 cDNA/B-肌动蛋白cDNA）显著降低（P<0.05）；与SI组（0.278±0.019）g/cm²和Ca组（0.302±0.026）g/cm²相比，SI + Ca组（0.231±0.025）g/cm²的肝脏组织IGF-１ mRNA含量显著降低（P<0.05）。
结论
大豆异黄酮联合补充钙比单独使用大豆异黄酮或补充钙饮食对股骨骨密度的丢失更具保护作用。SI剂量（37.95 mg/kg体重）可能显著抑制卵巢切除引起的肝脏组织IGF-1 mRNA含量的升高。
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