Gasslander T, Smeds S, Blomqvist L, Ihse I
Dept. of Surgery, University Hospital, Linköping, Sweden.
Scand J Gastroenterol. 1990 Nov;25(11):1111-7. doi: 10.3109/00365529008998542.
The effect of graded doses of secretin on exocrine pancreatic growth and cell proliferation was studied in a long-term experiment. After 16.5 and 150 micrograms/kg/24 h of secretin, each administered as two subcutaneous injections daily for 14 days, the pancreatic wet weight decreased, whereas the protein and DNA content of the gland was uninfluenced. When administered as described above for 14 days, 16.5, 50, 150, and 450 micrograms/kg/24 h of secretin did not affect the proliferation rate of ductal, acinar, or centroacinar cells as measured by a labeling index after 7 days of continuous 3H-thymidine administration. In the control groups a higher labeling index was found for ductal cells (19.9%) than for acinar cells (11.0%). During the 7 days of 3H-thymidine administration 80-90% of ductal and acinar cells remained in the G0 phase. There was a significantly higher labeling index in interlobular than in intralobular duct cells.
在一项长期实验中，研究了不同剂量的促胰液素对胰腺外分泌生长和细胞增殖的影响。以每天两次皮下注射的方式，连续14天给予16.5微克/千克/24小时和150微克/千克/24小时的促胰液素后，胰腺湿重下降，而胰腺的蛋白质和DNA含量未受影响。按上述方式给药14天，16.5微克/千克/24小时、50微克/千克/24小时、150微克/千克/24小时和450微克/千克/24小时的促胰液素，在连续给予3H-胸腺嘧啶核苷7天后，通过标记指数测定，对导管、腺泡或中央腺泡细胞的增殖率均无影响。在对照组中，导管细胞的标记指数（19.9%）高于腺泡细胞（11.0%）。在给予3H-胸腺嘧啶核苷的7天期间，80% - 90%的导管和腺泡细胞处于G0期。小叶间导管细胞的标记指数显著高于小叶内导管细胞。
