Gasslander T, Chu M, Smeds S, Ihse I
Dept. of Surgery, University Hospital, Linköping, Sweden.
Scand J Gastroenterol. 1991 Apr;26(4):399-404. doi: 10.3109/00365529108996501.
Pancreaticobiliary diversion (PBD) is known to induce chronic, endogenous hypercholecystokininemia causing pancreatic growth in rats. In the present study the proliferative response of the different exocrine pancreatic cells was studied by administration of 3H-thymidine, 1 mCi/kg, given 1 h before the rats were killed and 5, 10, 20, and 40 days after PBD. DNA and 3H-thymidine uptake, both expressed per 1 mg of pancreatic tissue, were significantly increased on day 5. The nuclear labeling index was increased fivefold in both the acinar and ductal cell group. In the centroacinar cell group the labeling index was increased on day 10. In conclusion, we found that the proliferative activity after PBD occurred during the first 10 days and that the ductal cells were forced into proliferation to the same extent as the acinar ones. These findings are of interest for future studies of hormonal influences on the development of pancreatic carcinoma.
已知胰胆分流术（PBD）会引发慢性内源性高胆囊收缩素血症，导致大鼠胰腺生长。在本研究中，通过在大鼠处死前1小时以及PBD术后5、10、20和40天给予1 mCi/kg的3H-胸腺嘧啶核苷，研究了不同胰腺外分泌细胞的增殖反应。以每1 mg胰腺组织计算的DNA和3H-胸腺嘧啶核苷摄取量在第5天显著增加。腺泡和导管细胞组的核标记指数增加了五倍。在中央腺泡细胞组中，标记指数在第10天增加。总之，我们发现PBD术后的增殖活性在前10天出现，并且导管细胞被迫增殖的程度与腺泡细胞相同。这些发现对于未来研究激素对胰腺癌发展的影响具有重要意义。
