Kajikawa K, Yasui W, Sumiyoshi H, Yoshida K, Nakayama H, Ayhan A, Yokozaki H, Ito H, Tahara E
First Department of Pathology, Hiroshima University School of Medicine, Japan.
Virchows Arch A Pathol Anat Histopathol. 1991;418(1):27-32. doi: 10.1007/BF01600241.
The expression of epidermal growth factor (EGF) was examined on various human tissues by radioimmunoassay, immunohistochemistry and Northern blot analysis. Immunoreactive EGF was found in most of the human tissues by radioimmunoassay at various levels. Large quantities of EGF were detected in the kidney and thyroid gland. Immunohistochemically, EGF immunoreactivity was detected mainly in the epithelial cells of the lung, stomach, duodenum, pancreas, kidney, pituitary gland, thyroid gland, mammary gland, ovary, uterus and placenta. Weakly EGF-positive cells were also found in the adrenal gland. The results of EGF-immunostaining were not always consistent with the data from radioimmunoassay. We consider that the amount of EGF measured by radioimmunoassay reflects the density of EGF-positive cells in the tissues and the concentration of EGF in individual EGF-positive cells. Furthermore, EGF mRNA was expressed in the salivary gland, thyroid gland, mammary gland and kidney. It is thus evident that EGF is produced by a variety of human tissues. The kidney expressed exceptionally high levels of EGF mRNA which was about one-tenth of the expression in mouse submandibular gland, suggesting that most of EGF in the urine is produced and secreted by the epithelial cells of renal tubules.
通过放射免疫分析、免疫组织化学和Northern印迹分析，对多种人体组织中表皮生长因子（EGF）的表达进行了检测。通过放射免疫分析在大多数人体组织中均检测到不同水平的免疫反应性EGF。在肾脏和甲状腺中检测到大量的EGF。免疫组织化学检测发现，EGF免疫反应性主要存在于肺、胃、十二指肠、胰腺、肾脏、垂体、甲状腺、乳腺、卵巢、子宫和胎盘的上皮细胞中。在肾上腺中也发现了弱阳性的EGF细胞。EGF免疫染色的结果与放射免疫分析的数据并不总是一致。我们认为，放射免疫分析所测得的EGF量反映了组织中EGF阳性细胞的密度以及单个EGF阳性细胞中EGF的浓度。此外，EGF mRNA在唾液腺、甲状腺、乳腺和肾脏中表达。因此，很明显EGF是由多种人体组织产生的。肾脏中EGF mRNA的表达水平异常高，约为小鼠颌下腺表达量的十分之一，这表明尿液中的大部分EGF是由肾小管上皮细胞产生和分泌的。
