Arts J J C, Walschot L H B, Verdonschot N, Schreurs B W, Buma P
Orthopedic Research Laboratory, Department of Orthopedics, Radboud University Nijmegen Medical Centre, 6500 HB Nijmegen, The Netherlands.
J Biomed Mater Res B Appl Biomater. 2007 May;81(2):476-85. doi: 10.1002/jbm.b.30687.
Reconstruction of bone defects with impacted morsellized cancellous bone grafts (MCB) is a popular method. Because of a shortage of human bone, mixing with biomaterials may be attractive. Ceramics may be used as bone graft extenders. In this study, various volume mixtures of biphasic tri-calciumphosphate/hydroxyl-apatite (TCP-HA) granules (1.5-2 mm) with MCB were examined in a non-loaded defect model in rabbits. Direct post-operatively, many 10-150 microm TCP-HA particles were present due to impaction. Irrespective to the group, after 8 weeks, virtually all MCB was resorbed and most TCP-HA granules were osseous-integrated with newly formed bone. The cross-sectional areas of TCP-HA after 8 weeks was generally smaller compared with direct post-operatively and the number of small 10-150 microm TCP-HA particles seemed reduced. Macrophages and giant cells were sparse after 8 weeks. In conclusion, the osteoconductivity of various mixtures of MCB and TCP-HA granules is not dependent on the ratio of TCP-HA over MCB. The reduced number of the 10-150microm TCP-HA particles after 8 weeks, may suggest that a cellular mediated resorption process of TCP-HA granules took place. Based on these favorable biological findings subsequent in-vivo experiments are warranted in load-bearing conditions to investigate whether these findings hold for joint reconstruction purposes.
采用嵌压式碎松质骨移植（MCB）修复骨缺损是一种常用方法。由于人骨来源短缺，与生物材料混合使用可能具有吸引力。陶瓷可作为骨移植填充材料。在本研究中，在兔非负重骨缺损模型中检测了双相磷酸三钙/羟基磷灰石（TCP-HA）颗粒（1.5 - 2毫米）与MCB的不同体积混合比例。术后即刻，由于嵌压作用存在许多10 - 150微米的TCP-HA颗粒。无论组别如何，8周后，几乎所有的MCB均被吸收，大多数TCP-HA颗粒与新形成的骨实现骨整合。与术后即刻相比，8周后TCP-HA的横截面积普遍变小，且10 - 150微米的小TCP-HA颗粒数量似乎减少。8周后巨噬细胞和巨细胞稀少。总之，MCB与TCP-HA颗粒不同混合物的骨传导性不依赖于TCP-HA与MCB的比例。8周后10 - 150微米TCP-HA颗粒数量减少，可能表明TCP-HA颗粒发生了细胞介导的吸收过程。基于这些良好的生物学结果，有必要在负重条件下进行后续体内实验，以研究这些结果是否适用于关节重建。
