Ferrari C, Scandroglio R
Istituto di Istologia ed Embriologia Generale dell' Università di Parma.
Arch Ital Anat Embriol. 1990 Jan-Mar;95(1):45-52.
In the present study a pre-embedding immunoelectron microscopical method ideally suited to detect suspended cell surface-associated antigens, is described. In this method 5nm colloidal gold particles have been enlarged by means of silver enhancement yielding a large marker that is easily detectable at the TEM level. The present method is particularly suited when investigation are performed with a low percentage of labeled cells as well as low antigen expression.
在本研究中,描述了一种预包埋免疫电子显微镜方法,该方法非常适合检测悬浮细胞表面相关抗原。在这种方法中,5nm的胶体金颗粒通过银增强进行放大,产生一个大的标记物,在透射电子显微镜(TEM)水平上很容易检测到。当对标记细胞百分比低以及抗原表达低的情况进行研究时,本方法特别适用。