Russo Thiago Luiz, Peviani Sabrina Messa, Freria Camila Marques, Gigo-Benato Davilene, Geuna Stefano, Salvini Tania Fátima
Department of Physical Therapy, Federal University of São Carlos, Rodovia Washington Luís, Km 235, CEP: 13565-905, São Carlos, São Paulo, Brazil.
Muscle Nerve. 2007 Jan;35(1):87-97. doi: 10.1002/mus.20668.
Denervation induces muscle fiber atrophy and changes in the gene expression rates of skeletal muscle. Electrical stimulation (ES) is a procedure generally used to treat denervated muscles in humans. This study evaluated the effect of ES based on chronaxie and rheobase on the expression of the myoD and atrogin-1 genes in denervated tibialis anterior (TA) muscle of Wistar rats. Five groups were examined: (1) denervated (D); (2) D+ES; (3) sham denervation; (4) normal (N); and (5) N+ES. Twenty muscle contractions were stimulated every 48 h using surface electrodes. After 28 days, ES significantly decreased the expression of myoD and atrogin-1 in D+ES compared to the D group. However, ES did not prevent muscle-fiber atrophy after denervation. Thus, ES based on chronaxie values and applied to denervated muscles using surface electrodes, as normally used in human rehabilitation, was able to reduce the myoD and atrogin-1 gene expressions, which are related to muscular growth and atrophy, respectively. The results of this study provide new information for the treatment of denervated skeletal muscle using surface ES.
去神经支配会导致肌纤维萎缩以及骨骼肌基因表达率的改变。电刺激（ES）是一种常用于治疗人类去神经支配肌肉的方法。本研究评估了基于时值和基强度的电刺激对Wistar大鼠去神经支配的胫骨前肌（TA）中肌分化抗原（myoD）和肌肉萎缩相关基因1（atrogin-1）基因表达的影响。研究了五组：（1）去神经支配组（D）；（2）去神经支配+电刺激组（D+ES）；（3）假去神经支配组；（4）正常组（N）；以及（5）正常+电刺激组（N+ES）。每隔48小时使用表面电极刺激20次肌肉收缩。28天后，与D组相比，电刺激显著降低了D+ES组中myoD和atrogin-1的表达。然而，电刺激并不能防止去神经支配后的肌纤维萎缩。因此，基于时值值并使用表面电极应用于去神经支配肌肉的电刺激，如同人类康复中通常使用的那样，能够降低分别与肌肉生长和萎缩相关的myoD和atrogin-1基因的表达。本研究结果为使用表面电刺激治疗去神经支配的骨骼肌提供了新的信息。
