Fan Xue-yun, Yan Zhao-feng, Yan Jin-de, Zhang Shu-jie, Bai Yu-Ping, Yao San-qiao
Department of Occupational Health and Environmental Health, North China Coal Medical College, Tangshan, Hebei Province 063000, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006 Sep;24(9):526-30.
To explore the relationship between the polymorphism of Interleukin-1 and the pneumoconiosis susceptibility.
Eighty patients with silicosis and 45 with coal workers' pneumoconiosis (CWP) were selected while 125 male workers, Han nationality in the same workplace as the patients were selected as the controls. Between the patients and the control, the differences of age and cumulative length of service were less than five years and two years, respectively. The controls were exposed to dusts but did not suffer from pneumoconiosis. Moreover, the patients and the controls were paired by 1:1. DNA was extracted from leucocytes by the hydroxybenzene chloroform method. The polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) techniques and PCR were used to examine polymorphism of IL-1alpha (-889), IL-1beta (-511) and IL-1Ra (+2018) and variable number of tandem repeats (VNTR) of IL-1Ra. After the preliminary experiment, the most adaptive PCR reaction, the restriction enzyme digest and electrophoresis system were used.
The difference in IL-1alpha (-889) 1/2 + 2/2 between the pneumoconiosis patients and the controls was significant (P < 0.01). The result of conditional Logistic regression showed that heterozygote and allele 2 of IL-1a (-889) were risk factors of pneumoconiosis. The difference in the genotype frequencies of IL-1beta (-511) 1/2 + 2/2, IL-1Ra (+2018) 1/2 + 2/2 and IL-Ra VNTR1/2 + 2/2 between the patients and the controls were not statistically significant (P > 0.05).
IL-1alpha (-889) gene polymorphism is related to pneumoconiosis. Workers with IL-1alpha (-889) allele 2 are susceptible to the pneumoconiosis. The relationship between IL-1beta (-511), IL-1Ra (+2018), IL-1Ra VNTR genes polymorphisms and pneumoconiosis are not found.
探讨白细胞介素-1基因多态性与尘肺病易感性之间的关系。
选取80例矽肺患者和45例煤工尘肺（CWP）患者，同时选取与患者在同一工作场所的125名汉族男性工人作为对照。患者与对照之间年龄和累计工龄差异分别小于5岁和2年。对照者接触粉尘但未患尘肺病。此外，患者与对照按1:1配对。采用酚氯仿法从白细胞中提取DNA。应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术和PCR检测白细胞介素-1α（-889）、白细胞介素-1β（-511）和白细胞介素-1受体拮抗剂（IL-1Ra）（+2018）的多态性以及IL-1Ra的可变串联重复序列（VNTR）。经过预实验后，采用最适宜的PCR反应、限制性酶切和电泳系统。
尘肺病患者与对照之间白细胞介素-1α（-889）1/2 + 2/2差异有统计学意义（P < 0.01）。条件Logistic回归结果显示，白细胞介素-1α（-889）杂合子和等位基因2是尘肺病的危险因素。患者与对照之间白细胞介素-1β（-511）1/2 + 2/2、白细胞介素-1Ra（+2018）1/2 + 2/2和白细胞介素-1Ra VNTR 1/2 + 2/2基因型频率差异无统计学意义（P > 0.05）。
白细胞介素-1α（-889）基因多态性与尘肺病有关。携带白细胞介素-1α（-889）等位基因2的工人易患尘肺病。未发现白细胞介素-1β（-511）、白细胞介素-1Ra（+2018）、白细胞介素-1Ra VNTR基因多态性与尘肺病之间的关系。
