Xing Jing-cai, Chen Wei-hong, Wang Feng, Han Wei-hui, Ren Hou-mao, Wu Tang-chun
MOE Key Lab of Environmental and Health, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006 Sep;24(9):540-3.
To assess the expression of Hsp72 and Hsp73 in chronic obstructive pulmonary disease (COPD) and to evaluate their roles in damage from coal dust exposure.
A case control study of 50 coal miners suffering from COPD and 50 healthy coal miners were selected from one coal mine. The levels of Hsp72 and Hsp73 in peripheral blood lymphocytes were determined by flow cytometry for all subjects.
(1) The expression of basic Hsp72 of peripheral blood lymphocytes for patients and controls was not different from that inducible expressed Hsp72 by 42 degrees C heat stress or by BPDE exposure. (2) The expression of Hsp72 in COPD patients (17.7 +/- 4.9) was significantly lower than that in healthy coal miners (22.6 +/- 10.0) (P < 0.01). On the other hand, the expression of Hsp73 in COPD patients (33.5 +/- 11.7) was higher than that in healthy coal miners (19.6 +/- 5.9) (P < 0.01). (3) A-positive relationship between the expression of Hsp72 and cumulative inhaling coal dust exposure was observed. No relationship was found between Hsp73 and cumulative inhaling coal dust exposure.
The decreased expressions of Hsp72 in peripheral blood lymphocytes of COPD coal miners.
评估热休克蛋白72（Hsp72）和热休克蛋白73（Hsp73）在慢性阻塞性肺疾病（COPD）中的表达，并评价它们在煤尘暴露所致损伤中的作用。
从某煤矿选取50例患COPD的煤矿工人和50例健康煤矿工人进行病例对照研究。采用流式细胞术测定所有研究对象外周血淋巴细胞中Hsp72和Hsp73的水平。
（1）患者和对照外周血淋巴细胞中基础Hsp72的表达与42℃热应激或苯并[a]芘二醇环氧化物（BPDE）暴露诱导表达的Hsp72无差异。（2）COPD患者Hsp72的表达（17.7±4.9）显著低于健康煤矿工人（22.6±10.0）（P<0.01）。另一方面，COPD患者Hsp73的表达（33.5±11.7）高于健康煤矿工人（19.6±5.9）（P<0.01）。（3）观察到Hsp72的表达与累积吸入煤尘暴露呈正相关。未发现Hsp73与累积吸入煤尘暴露之间存在关联。
COPD煤矿工人外周血淋巴细胞中Hsp72表达降低。
