Geist M J, Maris D O, Grady M S
Department of Neurological Surgery, University of Washington School of Medicine, Seattle 98104.
Exp Neurol. 1991 Feb;111(2):166-74. doi: 10.1016/0014-4886(91)90003-u.
Alterations in blood-brain barrier (BBB) function after brain grafting seem dependent on the donor phenotype and possibly on the grafting technique. Intracerebral blood grafts of nonneural tissue permanently disrupt the host BBB, while fetal neural block grafts probably do not. Cell suspensions, an alternative technique in brain grafting, disrupt the extracellular matrix of the graft. Fetal cell suspension allografts appear to form a functional BBB. We confirm and extend this finding to include fetal neural xenografts. Allograft and xenograft fetal neural cell suspensions were intracerebrally injected, and the BBB was examined using intravenous horseradish peroxidase (HRP). Neither graft type showed disruption of the BBB at the graft site from 2 weeks to more than 6 months after grafting. Vascular supply was prominent at all time points. Xenograft survival was improved with cyclosporine, yet cyclosporine did not affect BBB permeability. Cyclosporine did not interfere with repair of the BBB after simple brain trauma was induced by a control injection of saline. We conclude that fetal allograft and xenograft neural cell suspensions rapidly form and maintain a BBB impermeable to intravenous HRP.
脑移植后血脑屏障（BBB）功能的改变似乎取决于供体表型，也可能取决于移植技术。非神经组织的脑内血移植会永久性破坏宿主的血脑屏障，而胎儿神经块移植可能不会。细胞悬液作为脑移植的另一种技术，会破坏移植组织的细胞外基质。胎儿细胞悬液同种异体移植似乎能形成功能性血脑屏障。我们证实并扩展了这一发现，将胎儿神经异种移植也纳入其中。将同种异体移植和异种移植的胎儿神经细胞悬液注入脑内，并用静脉注射辣根过氧化物酶（HRP）检测血脑屏障。两种移植类型在移植后2周直至6个月以上，移植部位的血脑屏障均未出现破坏。在所有时间点，血管供应都很显著。环孢素可提高异种移植的存活率，但环孢素并不影响血脑屏障的通透性。在通过注射生理盐水诱导单纯脑外伤后，环孢素并不干扰血脑屏障的修复。我们得出结论，胎儿同种异体移植和异种移植神经细胞悬液能迅速形成并维持对静脉注射HRP不可渗透的血脑屏障。
