Akalan N, Grady M S
Department of Neurosurgery, Hacettepe University, Ankara, Turkey.
Surg Neurol. 1994 Dec;42(6):517-22. doi: 10.1016/0090-3019(94)90082-5.
Solid and suspension grafts of fetal central nervous system (CNS) tissue rapidly reform an intact blood-brain barrier (BBB), whereas solid grafts of peripheral nervous system (PNS) tissue fail to establish a BBB as detected by horseradish peroxide (HRP) leakage, administrated intravenously. We examined the acute changes in the BBB after grafting of fetal CNS tissue in solid and suspension form and superior cervical ganglion (SCG) and PNS tissue in the same manner. Adult rats (n = 20) received fetal (day 14-15) forebrain grafts (either solid or cell suspension) to their rostral corpus callosum bilaterally. A second group (n = 20) received SCG solid and cell suspension grafts at the same coordinates with the same technique. The animals were killed on first, third, seventh, and tenth days after grafting. Intravenous HRP (Sigma, type VI, 75 mg/5-g rat) was given 1 hour before perfusion with mixed aldehydes. Fifty-micron coronal sections were examined for the presence and location of the graft by cresyl violet and AChE staining and Mesulam's TMB method to detect HRP leakage. HRP leakage was detected in the parenchyma in all groups on the first and the third days post-transplantation indicating a disrupted BBB. No HRP reaction was seen at days 7 and 10 in groups receiving fetal forebrain tissue whether solid or cell suspension. Solid grafts of SCG consistently demonstrated HRP leakage from the first through the tenth day. However, cell suspension of SCG established a BBB by 7 days. These results suggest that within the solid grafts of CNS and PNS tissue, the permeability of the vessels is dictated by the transplanted tissue itself. When cell suspensions of the same tissue are introduced, host CNS tissue dominates as the local environment resulting in non-leaky vasculature within the graft.
胎儿中枢神经系统（CNS）组织的实体移植和悬浮移植能够迅速重建完整的血脑屏障（BBB），而外周神经系统（PNS）组织的实体移植在静脉注射辣根过氧化物酶（HRP）后检测到未能建立血脑屏障。我们以相同方式检查了实体和悬浮形式的胎儿CNS组织以及颈上神经节（SCG）和PNS组织移植后血脑屏障的急性变化。成年大鼠（n = 20）双侧在其胼胝体嘴部接受胎儿（第14 - 15天）前脑移植（实体或细胞悬浮液）。第二组（n = 20）以相同技术在相同坐标处接受SCG实体和细胞悬浮移植。在移植后的第1、3、7和10天处死动物。在灌注混合醛前1小时静脉注射HRP（Sigma，VI型，75mg/5g大鼠）。通过甲酚紫和乙酰胆碱酯酶染色以及Mesulam的TMB方法检查50微米的冠状切片，以检测移植体的存在和位置以及HRP渗漏。移植后第1天和第3天，所有组的实质中均检测到HRP渗漏，表明血脑屏障被破坏。接受胎儿前脑组织（无论是实体还是细胞悬浮液）的组在第7天和第10天未观察到HRP反应。SCG的实体移植从第1天到第10天一直显示HRP渗漏。然而，SCG细胞悬浮液在7天时建立了血脑屏障。这些结果表明，在CNS和PNS组织的实体移植中，血管的通透性由移植组织本身决定。当引入相同组织的细胞悬浮液时，宿主CNS组织作为局部环境起主导作用，导致移植体内血管无渗漏。
