Koda A, Miura T, Inagaki N, Sakamoto O, Arimura A, Nagai H, Mori H
Department of Pharmacology, Gifu Pharmaceutical University, Japan.
Int Arch Allergy Appl Immunol. 1990;92(3):209-16. doi: 10.1159/000235179.
Homologous passive cutaneous anaphylaxis (PCA) was induced by IgE antibody and, simultaneously, cutaneous reactions were induced by some allergic mediators such as histamine, serotonin and leukotriene (LT) C4 on rat back skin. Disodium cromoglycate and tranilast with inhibitory actions on mediator release inhibited PCA specifically, whereas antihistaminics, including ketotifen, azelastine, mequitazine and diphenhydramine, inhibited histamine- and serotonin-induced cutaneous reactions as well as PCA. Anti-slow-reacting substance of anaphylaxis drugs, KC-404 and FPL-55712, significantly inhibited PCA and histamine- and serotonin-induced reactions, but at the same doses they did not produce significant inhibition of the LTC4-induced reaction. All reactions tested were strongly inhibited dose dependently with the beta stimulants, salbutamol and isoproterenol, and a xanthine derivative, theophylline, which are known to increase the intracellular cyclic AMP level. We think that this method enables the determination of the properties of anti-allergic drugs.
通过IgE抗体诱导同源被动皮肤过敏反应（PCA），同时，通过组胺、5-羟色胺和白三烯（LT）C4等一些过敏介质在大鼠背部皮肤诱导皮肤反应。对介质释放有抑制作用的色甘酸钠和曲尼司特特异性地抑制PCA，而包括酮替芬、氮卓斯汀、美喹他嗪和苯海拉明在内的抗组胺药则抑制组胺和5-羟色胺诱导的皮肤反应以及PCA。过敏反应迟缓反应物质拮抗剂KC-404和FPL-55712显著抑制PCA以及组胺和5-羟色胺诱导的反应，但在相同剂量下它们对LTC4诱导的反应没有产生显著抑制作用。所有测试反应均被β激动剂沙丁胺醇和异丙肾上腺素以及已知可提高细胞内环磷酸腺苷水平的黄嘌呤衍生物茶碱强烈地剂量依赖性抑制。我们认为这种方法能够确定抗过敏药物的特性。
