Shimoda Kei, Akagi Masaaki, Hamada Hiroki
Department of Chemistry, Faculty of Medicine, Oita University, 1-1 Hasama-machi, Oita 879-5593, Japan.
Molecules. 2009 Aug 20;14(8):3106-14. doi: 10.3390/molecules14083106.
The glycosylation of alpha- and delta-tocopherols using Klebsiella pneumoniae and cyclodextrin glucanotransferase (CGTase) was investigated. K. pneumoniae converted alpha- and delta-tocopherols into the corresponding beta-glucosides in 10 and 8% yield, respectively. CGTase glycosylated alpha-tocopheryl beta-glucoside to alpha-tocopheryl beta-maltoside (51%) and alpha-tocopheryl beta-maltotrioside (35%). On the other hand, delta-tocopheryl beta-glucoside was converted into the corresponding beta-maltoside (45%) and beta-maltotrioside (29%) by CGTase. The beta-glucoside of alpha-tocopherol, and beta-glucoside and beta-maltoside of delta-tocopherol showed inhibitory effects on IgE antibody production and on histamine release from rat peritoneal mast cells.
研究了利用肺炎克雷伯菌和环糊精葡糖基转移酶（CGTase）对α-生育酚和δ-生育酚进行糖基化反应。肺炎克雷伯菌分别以10%和8%的产率将α-生育酚和δ-生育酚转化为相应的β-葡萄糖苷。CGTase将α-生育酚β-葡萄糖苷糖基化为α-生育酚β-麦芽糖苷（51%）和α-生育酚β-麦芽三糖苷（35%）。另一方面，CGTase将δ-生育酚β-葡萄糖苷转化为相应的β-麦芽糖苷（45%）和β-麦芽三糖苷（29%）。α-生育酚的β-葡萄糖苷以及δ-生育酚的β-葡萄糖苷和β-麦芽糖苷对IgE抗体产生和大鼠腹腔肥大细胞组胺释放具有抑制作用。
