[采用多重聚合酶链反应对WTK1细胞系进行tk基因突变分析]
[The tk gene mutation analysis in WTK1 cell line by multiplex PCR].
作者信息
Wang Yi-jing, Shuai Pei-qiang, Zhang Li-shi
机构信息
Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Sep;37(5):790-3.
OBJECTIVE
To explore the tk gene mutation spectrum in spontaneous and induced WTK1 mutants.
METHODS
After exposure to methyl methanesulfonate (MMS), mitomycin (MMC) or sodiam azide (NaN3), spontaneously-arising and induced mutants of WTK1 cell line were selected. The spectrum and hotspot of tk gene mutation were analyzed by multiplex PCR.
RESULTS
Three chemicals used in this study induced tk mutation frequency (MF) to increase significantly in a dose-dependent manner; most of the analyzed mutants had lost exon 4 and exon 5-7.
CONCLUSION
Three chemicals have mutagenic effect on WTK1 cell line, and obvious hotspot exists in tk gene mutation.
目的
探索自发和诱导的WTK1突变体中tk基因突变谱。
方法
用甲磺酸甲酯（MMS）、丝裂霉素（MMC）或叠氮化钠（NaN3）处理后，筛选WTK1细胞系的自发产生和诱导的突变体。通过多重PCR分析tk基因突变的谱和热点。
结果
本研究中使用的三种化学物质诱导tk突变频率（MF）以剂量依赖方式显著增加；大多数分析的突变体缺失了外显子4和外显子5 - 7。
结论
三种化学物质对WTK1细胞系有诱变作用，且tk基因突变存在明显热点。
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