[胸苷激酶基因座突变检测：L5178Y和TK6细胞系的比较]
[TK locus mutation assay: comparison of L5178Y and TK6 cell lines].
作者信息
Shuai Pei-qiang, Zhang Li-shi, Wang Yi-jing
机构信息
Department of Nutrition and Food Hygiene, West China School of Public Health, Sichuan, University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2004 Sep;35(5):650-3.
OBJECTIVE
To compare the advantages and disadvantages of utilizing L5178Y and TK6 cell lines in the TK locus mutation assay.
METHODS
The two cell lines were used for detecting and assessing the mutability of four chemicals (MMS, EMS, MMC and KCl); the microwell method of TK locus mutation assay was adopted.
RESULTS
The two cell lines brought about similar results in the study. The tolerance of TK6 to all four chemicals was lower than that of L5178Y, and all the relative mutation indices of TK6 were higher than those of L5178Y.
CONCLUSION
Each of the two cell lines has its own strong points; nevertheless, the authors recommend the applications of TK6 cell in the assay system since this cell line comes from human.
目的
比较在TK位点突变试验中使用L5178Y和TK6细胞系的优缺点。
方法
使用这两种细胞系检测和评估四种化学物质（甲基磺酸甲酯、乙基磺酸甲酯、丝裂霉素C和氯化钾）的致突变性；采用TK位点突变试验的微孔法。
结果
在该研究中，这两种细胞系产生了相似的结果。TK6对所有四种化学物质的耐受性均低于L5178Y，且TK6的所有相对突变指数均高于L5178Y。
结论
这两种细胞系各有优点；然而，作者推荐在试验系统中应用TK6细胞，因为该细胞系来源于人类。
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