Broxmeyer H E, Hangoc G, Cooper S, Anderson D, Cosman D, Lyman S D, Williams D E
Department of Medicine (Hematology/Oncology), Indiana University School of Medicine, Indianapolis 46202-5121.
Exp Hematol. 1991 Feb;19(2):143-6.
Purified natural and recombinant murine mast cell growth factor (MGF, a c-kit ligand) were evaluated alone and in combination with other cytokines for effects in vitro on colony formation by multipotential (CFU-GEMM), erythroid (BFU-E) and granulocyte-macrophage (CFU-GM) progenitor cells from BDF1 mouse bone marrow. Both preparations stimulated Epo-dependent CFU-GEMM and enhanced Epo-dependent BFU-E colony numbers and size. MGF had some stimulating activity for CFU-GM. When used in combination with plateau concentrations of pokeweed mitogen mouse spleen cell conditioned medium or granulocyte-macrophage colony stimulating factor (CSF), MGF enhanced in greater than additive fashion colony formation by CFU-GM. MGF also enhanced the size of colonies formed, an enhancement greatest for colonies containing granulocytes and macrophages. MGF did not enhance Macrophage-CSF stimulated colony numbers or size. MGF seems to be an early acting cytokine with preferential effects on the growth of more immature hematopoietic progenitor cells.
对纯化的天然和重组小鼠肥大细胞生长因子（MGF，一种c-kit配体）进行了单独评估，并与其他细胞因子联合评估其对BDF1小鼠骨髓中多能（CFU-GEMM）、红系（BFU-E）和粒细胞-巨噬细胞（CFU-GM）祖细胞体外集落形成的影响。两种制剂均刺激了依赖促红细胞生成素（Epo）的CFU-GEMM，并增加了依赖Epo的BFU-E集落数量和大小。MGF对CFU-GM有一定的刺激活性。当与商陆有丝分裂原小鼠脾细胞条件培养基或粒细胞-巨噬细胞集落刺激因子（CSF）的平台浓度联合使用时，MGF以大于相加的方式增强了CFU-GM的集落形成。MGF还增加了所形成集落的大小，对含有粒细胞和巨噬细胞的集落增加最大。MGF并未增加巨噬细胞集落刺激因子刺激的集落数量或大小。MGF似乎是一种早期作用的细胞因子，对更不成熟的造血祖细胞的生长具有优先作用。
