Migliaccio A R, Migliaccio G, Durand B, Mancini G C, Adamson J W
New York Blood Center, NY 10021.
Cytotechnology. 1993;11(2):107-13. doi: 10.1007/BF00748999.
We have analyzed the effect of stem cell factor (SCF), alone or in combination with other growth factors, on the generation of colony-forming cells (CFC) and on the expansion of hematopoiesis in vitro from light density, soybean agglutinin-, CD34+ cord blood cells under serum-deprived conditions. The growth factors were either added only once at the onset of the culture or added every few days when the cultures were demidepopulated and refed with fresh medium. No growth factor, alone, generated CFC or expanded hematopoiesis under these conditions. However, SCF, in combination with interleukin 3 (IL-3) or with "late-acting factors" (granulocyte colony-stimulating factor (G-CSF) or erythropoietin (Epo)), generated large numbers of mature cells as well as CFC. The number of CFC generated depended on the refeeding procedure adopted. In cultures never refed, the CFC numbers increased from < 160 CFC/culture at day 0 to > 3000 CFC at day 10. The CFC numbers stayed above the input levels for 25 days before declining. Almost no CFC were detectable after one month. In contrast, in cultures regularly refed, CFC were detectable for at least 40 days. The lineages of the mature cells and the types of CFC generated varied with the different growth factors. In the presence of SCF plus IL-3, erythroid burst-forming cells (BFU-E) and granulocyte/macrophage colony-forming cells (GM-CFC) were generated and erythroid as well as myelomonocytic precursors were present among the differentiated cells.(ABSTRACT TRUNCATED AT 250 WORDS)
我们分析了干细胞因子（SCF）单独或与其他生长因子联合使用，对低密度、大豆凝集素、CD34⁺脐血细胞在血清剥夺条件下体外生成集落形成细胞（CFC）以及造血扩增的影响。生长因子要么在培养开始时仅添加一次，要么在培养物细胞数量减少并更换新鲜培养基时每隔几天添加一次。在这些条件下，单独使用任何生长因子都不会生成CFC或扩增造血。然而，SCF与白细胞介素3（IL-3）或“后期作用因子”（粒细胞集落刺激因子（G-CSF）或促红细胞生成素（Epo））联合使用时，会生成大量成熟细胞以及CFC。生成的CFC数量取决于所采用的换液程序。在从未换液的培养物中，CFC数量从第0天的<160个CFC/培养物增加到第10天的>3000个CFC。CFC数量在下降前25天一直高于输入水平。一个月后几乎检测不到CFC。相比之下，在定期换液的培养物中，CFC至少40天可检测到。成熟细胞的谱系和生成的CFC类型因不同的生长因子而异。在存在SCF加IL-3的情况下，会生成红系爆式集落形成细胞（BFU-E）和粒细胞/巨噬细胞集落形成细胞（GM-CFC），并且在分化细胞中存在红系以及髓单核系前体细胞。（摘要截断于250字）
