Weng Wen, Gao Qin, Li Xiao-guang, Luo Huan-min
Jinan University, Guangzhou 510632, China.
Zhong Yao Cai. 2006 Jun;29(6):567-9.
To develop a method of RP-HPLC for determination of schisandrin content in Kadsura heteroclita (Roxb.) Craib.
RP-HPLC analysis was carried out by using Kromasil C18 column (5 microm, 250 mm x 4.6 mm) and methanol-water (65:35) as the mobile, and the detection wavelength was 250 nm.
The method was linear in the range of 0.08-0.06 nicrog (r = 0.9998), and the average recovery was 100.24%, with the RSD of 2.09% (n=5).
The method was simple, accurate, highly sensitive and reproducible. It may be used for the quantitative determination of schisandrin in extract of Kadsura heteroclita (Roxb.) Craib.
建立一种反相高效液相色谱法测定黑老虎中五味子醇甲的含量。
采用Kromasil C18柱（5μm，250mm×4.6mm），以甲醇-水（65:35）为流动相进行反相高效液相色谱分析，检测波长为250nm。
该方法在0.08 - 0.06μg范围内线性关系良好（r = 0.9998），平均回收率为100.24%，相对标准偏差为2.09%（n = 5）。
该方法简便、准确、灵敏度高、重现性好，可用于黑老虎提取物中五味子醇甲的定量测定。
