[秦岭五味子属四种果实中木脂素的测定]
[Determination of lignans in four fruits of Schisandra genus in Qinling mountains].
作者信息
Liu Xiao-Xia, Liu Fang, Gu Wei
机构信息
Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, Xi'an, China.
National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, Xi'an, China.
出版信息
Zhong Yao Cai. 2013 Jul;36(7):1053-5.
OBJECTIVE
To determine the content of four lignans in the fruits of Schisandra sphenanthera, Schisandra grandiflora, Schisandra rubriflora and Schisandra propinqua subsp. sinensis.
METHODS
The HPLC separation was performed on Diamonsil C18 (150 mm x 4.6 mm, 5 microm) columm using methanol-water (70 : 30) as the mobile phase at a flow rate of 0.8 mL/min, with the detection wavelength of 280 nm.
RESULTS
Schisandrin, deoxyschizandrin and gamma-schizandrin were detected in four species of Schisandra. The content of schisantherin A in S. sphenanthera was measured up to the set standards of the Chinese Pharmacopoeia (2010 Edition). The content of deoxyschizandrin was high in S. grandiflora and S. rubriflora, and that of gamma-schizandrin was high in S. propinqua subsp. sinensis.
CONCLUSION
The content of lignans is different in four species of Schisandra fruits in Qinling Mountains, it provides scientific reference for further study.
目的
测定华中五味子、大花五味子、红花五味子和中华五味子亚种果实中4种木脂素的含量。
方法
采用Diamonsil C18柱（150 mm×4.6 mm，5μm），以甲醇-水（70∶30）为流动相，流速为0.8 mL/min，检测波长为280 nm，进行高效液相色谱分离。
结果
在4种五味子中均检测到五味子醇甲、脱氧五味子素和γ-五味子素。华中五味子中五味子酯甲的含量达到《中国药典》（2010年版）规定标准。大花五味子和红花五味子中脱氧五味子素含量较高，中华五味子亚种中γ-五味子素含量较高。
结论
秦岭地区4种五味子果实中木脂素含量存在差异，为进一步研究提供了科学参考。
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