Westgate G E, Messenger A G, Watson L P, Gibson W T
Personal Products Research Section, Unilever Research, Bedford, U.K.
J Invest Dermatol. 1991 Feb;96(2):191-5. doi: 10.1111/1523-1747.ep12461019.
The involvement of proteoglycans in hair growth has been recognized through the observation of increased hair growth in diseases such as the mucopolysaccharidoses and pre-tibial myxedema, which involve an increase in skin proteoglycan content. In an attempt to understand this, we have examined the distribution of chondroitin 6 sulphate (C6S), unsulphated chondroitin (COS), dermatan sulphate (DS), and heparan sulphate proteoglycans (HSPG) in frozen tissue sections of normal scalp by immunostaining. Results show that during anagen, the thick connective tissue sheath around the follicle strains strongly for C6S, COS, and DS. COS is uniquely associated with this region and is not found beneath the epidermis or infundibular epithelium. HSPG is, however, localized in the basement membrane zone adjacent to the outer root sheath. In addition, all of these proteoglycans are localized in the dermal papilla. In mid-catagen, we observed significant loss of C6S and COS staining from both the dermal papilla and the connective tissue sheath, but no decrease in staining for HSPG. In late catagen, very little staining of C6S and COS was observed. In early anagen, we observed that C6S was again present in the connective tissue sheath and dermal papilla; however, COS staining appeared to be weaker and less closely associated with the follicle. HSPG staining was observed in early anagen in a pattern very similar to that found for other basement membrane components. Results for DS were not obtained for catagen or early anagen. These results provide further evidence that hair growth is associated with the presence of chondroitin proteoglycans in the follicle environment and that the cessation of growth is associated with their removal. Further studies are underway to characterize the relationship between hair growth and proteoglycans.
通过观察诸如黏多糖贮积症和胫前黏液性水肿等疾病中毛发生长增加的现象，人们认识到蛋白聚糖与毛发生长有关，这些疾病涉及皮肤蛋白聚糖含量的增加。为了理解这一点，我们通过免疫染色检查了正常头皮冷冻组织切片中硫酸软骨素6（C6S）、未硫酸化软骨素（COS）、硫酸皮肤素（DS）和硫酸乙酰肝素蛋白聚糖（HSPG）的分布。结果表明，在生长期，毛囊周围的厚结缔组织鞘对C6S、COS和DS有强烈染色。COS独特地与该区域相关联，在表皮或漏斗状上皮下方未发现。然而，HSPG定位于与外根鞘相邻的基底膜区。此外，所有这些蛋白聚糖都定位于真皮乳头。在退行期中期，我们观察到真皮乳头和结缔组织鞘中C6S和COS染色显著减少，但HSPG染色没有减少。在退行期末期，观察到C6S和COS的染色很少。在生长期早期，我们观察到C6S再次出现在结缔组织鞘和真皮乳头中；然而，COS染色似乎较弱，与毛囊的关联也不那么紧密。在生长期早期观察到HSPG染色的模式与其他基底膜成分非常相似。未获得退行期或生长期早期DS的结果。这些结果进一步证明，毛发生长与毛囊环境中软骨素蛋白聚糖的存在有关，而生长停止与它们的去除有关。正在进行进一步的研究以表征毛发生长与蛋白聚糖之间的关系。
