Yamane Y, Yaoita H, Couchman J R
Department of Dermatology, Jichi Medical School, Tochigi, Japan.
J Invest Dermatol. 1996 Mar;106(3):531-7. doi: 10.1111/1523-1747.ep12343940.
Basement membrane proteoglycans in mammalian skin comprise at least one chondroitin sulfate proteoglycan and heparan sulfate proteoglycans, including perlecan. In this study, the origins of basement membrane chondroitin sulfate proteoglycan and perlecan were investigated both in vivo and in vitro. For in vivo experiments, pieces of newborn rat epidermis obtained by dispase treatment were grafted onto athymic nude mice. Three and six weeks after grafting, immunofluorescence analysis of the grafted skin was carried out, using monoclonal antibodies specific for rat basement membrane chondroitin sulfate proteoglycan and rat and mouse perlecan. While the isolated rat epidermis was shown to completely lack rat basement membrane chondroitin sulfate proteoglycan and rat basement membrane heparan sulfate proteoglycans, including perlecan, immunofluorescence staining of tissue sections from the grafted sites on mice demonstrated the presence of rat basement membrane chondroitin sulfate proteoglycan and rat perlecan on interfollicular and follicular basement membranes including that separating dermal papillae from adjacent hair follicle epithelium. In contrast, the basement membranes of all dermal capillaries were positive for mouse perlecan, but negative for rat basement membrane chondroitin sulfate proteoglycan and rat perlecan, including the basement membranes of papillary dermal capillaries beneath the rat epidermis. These data suggest that basement membrane proteoglycans of the dermal-epidermal junction and hair follicle epithelium are of epidermal (epithelial) origin in vivo. Stratified rat keratinocytes cultured on a collagen matrix at the air-liquid interface showed the synthesis of perlecan, laminin 1, and type IV collagen in basement membranes, but not clearly detectable basement membrane chondroitin sulfate proteoglycan.
哺乳动物皮肤中的基底膜蛋白聚糖至少包括一种硫酸软骨素蛋白聚糖和硫酸乙酰肝素蛋白聚糖，其中硫酸乙酰肝素蛋白聚糖包括基底膜聚糖。在本研究中，对基底膜硫酸软骨素蛋白聚糖和基底膜聚糖在体内和体外的来源进行了研究。对于体内实验，通过dispase处理获得的新生大鼠表皮片被移植到无胸腺裸鼠身上。移植后三周和六周，使用针对大鼠基底膜硫酸软骨素蛋白聚糖以及大鼠和小鼠基底膜聚糖的单克隆抗体，对移植皮肤进行免疫荧光分析。虽然分离出的大鼠表皮被证明完全缺乏大鼠基底膜硫酸软骨素蛋白聚糖和大鼠基底膜硫酸乙酰肝素蛋白聚糖（包括基底膜聚糖），但对小鼠移植部位组织切片的免疫荧光染色显示，在包括将真皮乳头与相邻毛囊上皮分隔开的毛囊间和毛囊基底膜上，存在大鼠基底膜硫酸软骨素蛋白聚糖和大鼠基底膜聚糖。相比之下，所有真皮毛细血管的基底膜对小鼠基底膜聚糖呈阳性，但对大鼠基底膜硫酸软骨素蛋白聚糖和大鼠基底膜聚糖（包括大鼠表皮下方乳头真皮毛细血管的基底膜）呈阴性。这些数据表明，在体内，真皮 - 表皮交界处和毛囊上皮的基底膜蛋白聚糖来源于表皮（上皮）。在气液界面的胶原基质上培养的分层大鼠角质形成细胞在基底膜中显示出基底膜聚糖、层粘连蛋白1和IV型胶原的合成，但未明显检测到基底膜硫酸软骨素蛋白聚糖。
