[突尼斯成年1型糖尿病患者中乳糜泻血清学标志物的患病率]

[Prevalence of celiac disease serological markers in Tunisian type 1 diabetic adults].

作者信息

Laadhar L, Zitouni M, Kallel-Sellami M, Masmoudi S, Bouguerra R, Chaabouni H, Ben Slama C, Makni S

机构信息

Laboratoire d'Immunologie, Hôpital La Rabta, Tunis.

出版信息

Ann Biol Clin (Paris). 2006 Sep-Oct;64(5):439-44.

PMID:17040874

Abstract

OBJECTIVE

to evaluate the prevalence of celiac serological markers; anti-transglutaminase (ATGt), anti-endomysium (AE), anti-gliadin (AGD) and anti-reticulin (AR) antibodies; in type 1 diabetic Tunisian adults.

SUBJECTS AND METHODS

261 type 1 diabetic patients aged from 16 to 60 years were enrolled in this prospective study. IgG and IgA transglutaminase and gliadin were measured with ELISA. IgA AE were tested by indirect immunofluorescence using 2 substrates; monkey oesophagus and human umbilical cord. AR were detected by indirect immunofluorescence on rat liver, kidney and stomach. Sera IgA level was measured by turbidimetry.

RESULTS

83/261 of diabetics were positive for at least one antibody, 5.7% had ATGt-A, 3.4% AE on monkey esophagus, 3.1% AE on umbilical cordon, 18% AGD-A, 19.5% AGD-G and 3.1% AR. There was an excellent concordance between AE and ATGt (r = 0.9). Out of the 261 diabetics, 5 had an IgA deficiency and one of them has IgG AE and ATGt.

CONCLUSION

serological markers of celiac disease seem to be frequent in diabetics. Nevertheless, diagnosis must be confirmed by histological studies which allow us to know the real prevalence of celiac disease in diabetic adults.

摘要

目的

评估1型糖尿病突尼斯成年人中乳糜泻血清学标志物；抗转谷氨酰胺酶（ATGt）、抗肌内膜（AE）、抗麦醇溶蛋白（AGD）和抗网硬蛋白（AR）抗体的患病率。

对象与方法

261名年龄在16至60岁之间的1型糖尿病患者纳入了这项前瞻性研究。采用酶联免疫吸附测定法检测IgG和IgA转谷氨酰胺酶及麦醇溶蛋白。使用两种底物（猴食管和人脐带）通过间接免疫荧光法检测IgA AE。通过对大鼠肝脏、肾脏和胃进行间接免疫荧光法检测AR。采用比浊法测量血清IgA水平。

结果

83/261的糖尿病患者至少一种抗体呈阳性，5.7%有ATGt-A，3.4%在猴食管上有AE，3.1%在脐带上有AE，18%有AGD-A，19.5%有AGD-G，3.1%有AR。AE和ATGt之间有极好的一致性（r = 0.9）。在261名糖尿病患者中，5人有IgA缺乏，其中1人有IgG AE和ATGt。