Laadhar L, Zitouni M, Kallel-Sellami M, Masmoudi S, Bouguerra R, Chaabouni H, Ben Slama C, Makni S
Laboratoire d'Immunologie, Hôpital La Rabta, Tunis.
Ann Biol Clin (Paris). 2006 Sep-Oct;64(5):439-44.
to evaluate the prevalence of celiac serological markers; anti-transglutaminase (ATGt), anti-endomysium (AE), anti-gliadin (AGD) and anti-reticulin (AR) antibodies; in type 1 diabetic Tunisian adults.
261 type 1 diabetic patients aged from 16 to 60 years were enrolled in this prospective study. IgG and IgA transglutaminase and gliadin were measured with ELISA. IgA AE were tested by indirect immunofluorescence using 2 substrates; monkey oesophagus and human umbilical cord. AR were detected by indirect immunofluorescence on rat liver, kidney and stomach. Sera IgA level was measured by turbidimetry.
83/261 of diabetics were positive for at least one antibody, 5.7% had ATGt-A, 3.4% AE on monkey esophagus, 3.1% AE on umbilical cordon, 18% AGD-A, 19.5% AGD-G and 3.1% AR. There was an excellent concordance between AE and ATGt (r = 0.9). Out of the 261 diabetics, 5 had an IgA deficiency and one of them has IgG AE and ATGt.
serological markers of celiac disease seem to be frequent in diabetics. Nevertheless, diagnosis must be confirmed by histological studies which allow us to know the real prevalence of celiac disease in diabetic adults.
评估1型糖尿病突尼斯成年人中乳糜泻血清学标志物；抗转谷氨酰胺酶（ATGt）、抗肌内膜（AE）、抗麦醇溶蛋白（AGD）和抗网硬蛋白（AR）抗体的患病率。
261名年龄在16至60岁之间的1型糖尿病患者纳入了这项前瞻性研究。采用酶联免疫吸附测定法检测IgG和IgA转谷氨酰胺酶及麦醇溶蛋白。使用两种底物（猴食管和人脐带）通过间接免疫荧光法检测IgA AE。通过对大鼠肝脏、肾脏和胃进行间接免疫荧光法检测AR。采用比浊法测量血清IgA水平。
83/261的糖尿病患者至少一种抗体呈阳性，5.7%有ATGt-A，3.4%在猴食管上有AE，3.1%在脐带上有AE，18%有AGD-A，19.5%有AGD-G，3.1%有AR。AE和ATGt之间有极好的一致性（r = 0.9）。在261名糖尿病患者中，5人有IgA缺乏，其中1人有IgG AE和ATGt。
糖尿病患者中乳糜泻的血清学标志物似乎很常见。然而，诊断必须通过组织学研究来证实，这使我们能够了解糖尿病成年人中乳糜泻的实际患病率。
