Hellberg P, Brannstrom M
Department of Obstetrics and Gynecology, Sahlgrenska Sjukhuset, Göteborg, Sweden.
Prostaglandins. 1990 Oct;40(4):361-71. doi: 10.1016/0090-6980(90)90101-z.
Ovaries from immature rats, primed with pregnant mare's serum gonadotropin (PMSG; 20 IU, on day 28), were perfused in vitro in a recirculating system for 21 h from the morning of day 30 of age. Stimulation with luteinizing hormone (LH; 0.1 micrograms/ml) in vitro at 0 h of perfusion resulted in 2.4 +/- 0.75 (mean +/- SEM) ovulations per treated ovary, whereas no ovulations occurred in the unstimulated group. When the addition of LH was supplemented hourly for 10 h with a stable prostacyclin analogue, Iloprost, at concentrations of 0.01 microM or 0.1 microM, the ovulation rate increased significantly (p less than 0.05) to 6.6 +/- 1.3 and 10.2 +/- 2.4 ovulations per treated ovary, respectively. Iloprost (0.1 microM) did not cause any follicular ruptures when added by itself at every hour up to 10 h. The addition of Iloprost did not affect the release of cyclic adenosine 3',5'-monophosphate (cAMP), progesterone or estradiol from unstimulated or LH-stimulated ovaries. All ovulated oocytes had resumed meiosis as judged from the absence of a germinal vesicle. These data indicate a positive modulatory role of prostacyclin in the LH-induced ovulatory process for the rat.
用孕马血清促性腺激素（PMSG；20国际单位，第28天）预处理的未成熟大鼠卵巢，在30日龄上午开始于体外循环系统中灌注21小时。灌注0小时时，体外给予促黄体生成素（LH；0.1微克/毫升）刺激，每个处理的卵巢排卵2.4±0.75（平均值±标准误）次，而未刺激组未发生排卵。当在10小时内每小时补充稳定的前列环素类似物伊洛前列素，浓度为0.01微摩尔/升或0.1微摩尔/升时，排卵率显著增加（p<0.05），每个处理的卵巢分别排卵6.6±1.3次和10.2±2.4次。在长达10小时内每小时单独添加伊洛前列素（0.1微摩尔/升）不会引起任何卵泡破裂。添加伊洛前列素不影响未刺激或LH刺激的卵巢释放环磷酸腺苷（cAMP）、孕酮或雌二醇。从无生发泡判断，所有排卵的卵母细胞均已恢复减数分裂。这些数据表明前列环素在大鼠LH诱导的排卵过程中具有正向调节作用。
