Brännström M, Wang L, Norman R J
Department of Obstetrics and Gynecology, University of Adelaide, Queen Elizabeth Hospital, Woodville, South Australia.
Endocrinology. 1993 Jan;132(1):399-404. doi: 10.1210/endo.132.1.8419137.
The involvement of cytokines derived from ovarian and hematopoietic cells have been suggested in the cyclic events of the ovary. In the present study the effects of two cytokines, interleukin-1 beta (IL-1 beta) and IL-2, on ovulation, steroidogenesis, and prostaglandin (PG) production were explored in rat ovaries perfused in vitro. Ovaries of equine CG (20 IU)-primed immature rats were perfused in a recirculating system for 20 h, and samples were taken for analysis of progesterone, estradiol, prostaglandin E (PGE), and PGF2 alpha. The number of ovulations was estimated by counting the number of oocytes released into the ovarian bursae. Unstimulated ovaries did not ovulate, whereas the addition of LH (100 ng/ml) resulted in 3.4 +/- 0.6 ovulations/treated ovary. The addition of human recombinant IL-1 beta (4 ng/ml) induced ovulation (1.6 +/- 0.4) and increased the LH-induced ovulation rate 3-fold (9.8 +/- 0.5). The addition of human recombinant IL-2 (40 ng/ml) did not induce ovulation and did not affect the LH-induced ovulation rate. Ovarian release of progesterone and PGF2 alpha was increased by IL-1 beta, but estradiol and PGE release was not affected. IL-2 did not affect steroidogenesis or PG release. To elucidate whether the IL-1 beta-stimulated ovarian synthesis of PGF2 alpha was crucial for the ovulatory effect of IL-1 beta, experiments were performed in the presence of indomethacin (5 micrograms/ml), an inhibitor of PG synthesis. Indomethacin (5 micrograms/ml) inhibited LH-induced ovulation almost completely (one ovulation in one of the five treated ovaries), but did not affect the IL-1 beta-induced ovulation rate (1.4 +/- 0.2). The number of ovulations in the group treated with LH and IL-1 beta was significantly reduced (3.2 +/- 0.6) in the presence of indomethacin. These data demonstrate that IL-1 beta induces ovulation in the rat ovary, and this effect may be partly mediated by the increased production of the ovulatory mediator progesterone.
卵巢和造血细胞衍生的细胞因子参与卵巢的周期性活动已被提出。在本研究中，探讨了两种细胞因子，即白细胞介素-1β（IL-1β）和IL-2，对体外灌注的大鼠卵巢排卵、类固醇生成和前列腺素（PG）产生的影响。用马绒毛膜促性腺激素（20 IU）预处理的未成熟大鼠的卵巢在循环系统中灌注20小时，采集样本分析孕酮、雌二醇、前列腺素E（PGE）和前列腺素F2α。通过计数释放到卵巢囊中的卵母细胞数量来估计排卵数。未受刺激的卵巢不排卵，而添加促黄体生成素（LH，100 ng/ml）导致每个处理的卵巢有3.4±0.6个排卵。添加重组人IL-1β（4 ng/ml）诱导排卵（1.6±0.4），并使LH诱导的排卵率增加3倍（9.8±0.5）。添加重组人IL-2（40 ng/ml）不诱导排卵，也不影响LH诱导的排卵率。IL-1β增加了卵巢孕酮和PGF2α的释放，但不影响雌二醇和PGE的释放。IL-2不影响类固醇生成或PG释放。为了阐明IL-1β刺激卵巢合成PGF2α是否对IL-1β的排卵作用至关重要，在存在PG合成抑制剂吲哚美辛（5μg/ml）的情况下进行了实验。吲哚美辛（5μg/ml）几乎完全抑制LH诱导的排卵（五个处理的卵巢中有一个排卵），但不影响IL-1β诱导的排卵率（1.4±0.2）。在存在吲哚美辛的情况下，用LH和IL-1β处理的组中的排卵数显著减少（3.2±0.6）。这些数据表明，IL-1β在大鼠卵巢中诱导排卵，并且这种作用可能部分由排卵介质孕酮的产生增加介导。
