质粒编码的抗砷泵：一种阴离子转运ATP酶。

The plasmid-encoded arsenical resistance pump: an anion-translocating ATPase.

作者信息

Rosen B P

机构信息

Department of Biochemistry, Wayne State University, School of Medicine, Detroit, MI 48201.

出版信息

Res Microbiol. 1990 Mar-Apr;141(3):336-41. doi: 10.1016/0923-2508(90)90008-e.

DOI:10.1016/0923-2508(90)90008-e

PMID:1704144

Abstract

An anion-translocating ATPase has been identified as the product of the arsenical resistance operon of resistance plasmid R773. When expressed in Escherichia coli this ATP-driven oxyanion pump catalyses extrusion of the oxyanions arsenite, antimonite and arsenate. Maintenance of a low intracellular concentration of oxyanion produces resistance to the toxic agents. The pump is composed of two polypeptides, the products of the arsA and arsB genes. This two-subunit enzyme produces resistance to arsenite and antimonite. A third gene, arsC, expands the substrate specificity to allow for arsenate pumping and resistance.

摘要

一种阴离子转运ATP酶已被鉴定为耐药质粒R773的抗砷操纵子的产物。当在大肠杆菌中表达时，这种由ATP驱动的氧阴离子泵催化亚砷酸盐、亚锑酸盐和砷酸盐等氧阴离子的排出。维持细胞内低浓度的氧阴离子可产生对这些有毒剂的抗性。该泵由两种多肽组成，即arsA和arsB基因的产物。这种双亚基酶产生对亚砷酸盐和亚锑酸盐的抗性。第三个基因arsC扩大了底物特异性，以允许砷酸盐的泵出和抗性。