Ohhara N, Okamura S, Hayashi S, Shibuya T, Okada F, Ishikawa M, Hosokawa M, Kobayashi H, Niho Y
First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Res Exp Med (Berl). 1990;190(5):305-14. doi: 10.1007/BF00000037.
Murine fibrosarcoma cell line BMT-11 was induced with 3-methylcholanthrene and maintained in culture. Transplantation of BMT-11 into syngeneic C57BL/6 mice produced leukocytosis consisting of marked increments of neutrophils and monocytes associated with massive splenomegaly. In order to elucidate the mechanisms of this leukemoid reaction, we studied the changes occurring in hematopoietic progenitor cells in BMT-11-transplanted mice. The numbers of granulocyte-macrophage colony-forming units (CFU-GM), erythroid colony-forming units (CFU-E), erythroid burst-forming units (BFU-E), and mixed colony-forming units (CFU-Mix) in the spleen showed dramatic 216-fold, 18-fold, 64-fold, and 80-fold increases, respectively, relative to the value in the control mice 5 weeks after the BMT-11 implantation. In contrast, the levels of progenitor cells in the bone marrow remained within normal limits. The nature of the colony-stimulating factor (CSF) secreted from BMT-11 tumor cells was also studied. BMT-11-conditioned medium (BMT-11-CM), BMT-11 tumor extract, and sera from the mice bearing transplanted BMT-11 tumor contained CSF that stimulated mainly granulocyte and macrophage lineages. Furthermore, the expression of the granulocyte colony-stimulating factor (G-CSF) gene in BMT-11 cells were detected by Northern blot analysis.
小鼠纤维肉瘤细胞系BMT-11用3-甲基胆蒽诱导并在培养中维持。将BMT-11移植到同基因C57BL/6小鼠中产生白细胞增多症，包括中性粒细胞和单核细胞显著增加，并伴有巨大脾肿大。为了阐明这种类白血病反应的机制，我们研究了BMT-11移植小鼠造血祖细胞中发生的变化。与BMT-11植入后5周的对照小鼠相比，脾脏中粒细胞-巨噬细胞集落形成单位（CFU-GM）、红系集落形成单位（CFU-E）、红系爆式集落形成单位（BFU-E）和混合集落形成单位（CFU-Mix）的数量分别显著增加了216倍、18倍、64倍和80倍。相反，骨髓中祖细胞水平保持在正常范围内。还研究了BMT-11肿瘤细胞分泌的集落刺激因子（CSF）的性质。BMT-11条件培养基（BMT-11-CM）、BMT-11肿瘤提取物以及携带移植BMT-11肿瘤的小鼠血清中含有主要刺激粒细胞和巨噬细胞系的CSF。此外，通过Northern印迹分析检测了BMT-11细胞中粒细胞集落刺激因子（G-CSF）基因的表达。
