Halim Yuwana, Schwartz Steven J, Francis David, Baldauf Nathan A, Rodriguez-Saona Luis E
The Ohio State University, Department of Food Science and Technology, 110 Parker Food Science and Technology Bldg, 2015 Fyffe Rd, Columbus, OH 43210, USA.
J AOAC Int. 2006 Sep-Oct;89(5):1257-62.
Lycopene is a potent antioxidant that has been shown to play critical roles in disease prevention. Efficient assays for detection and quantification of lycopene are desirable as alternatives to time- and labor-intensive methods. Attenuated total reflectance infrared (ATR-IR) spectroscopy was used for quantification of lycopene in tomato varieties. Calibration models were developed by partial least-squares regression (PLSR) using quantitative measures of lycopene concentration from liquid chromatography as reference method. IR spectra showed a distinct marker band at 957 cm(-1) for trans Carbon-Hydrogen (CH) deformation vibration of lycopene. PLSR models predicted the lycopene content accurately and reproducibly with a correlation coefficient (sigma) of 0.96 and standard error of cross-validation <0.80 mg/100 g. ATR-IR spectroscopy allowed for rapid, simple, and accurate determination of lycopene in tomatoes with minimal sample preparation. Results suggest that the ATR-IR method is applicable for high-throughput quantitative analysis and screening for lycopene in tomatoes.
番茄红素是一种有效的抗氧化剂，已被证明在疾病预防中发挥关键作用。作为耗时且费力方法的替代方案，需要高效的番茄红素检测和定量分析方法。衰减全反射红外（ATR-IR）光谱法用于番茄品种中番茄红素的定量分析。通过偏最小二乘回归（PLSR）建立校准模型，使用液相色谱法测定的番茄红素浓度定量指标作为参考方法。红外光谱显示，在957 cm(-1)处有一个明显的特征峰，对应番茄红素反式碳-氢（CH）变形振动。PLSR模型预测番茄红素含量准确且可重复，相关系数（sigma）为0.96，交叉验证标准误差<0.80 mg/100 g。ATR-IR光谱法能够在样品制备最少的情况下快速、简单且准确地测定番茄中的番茄红素。结果表明，ATR-IR方法适用于番茄红素的高通量定量分析和筛选。
