Ilchmann D, Helbig D, Göhler H, Stopsack M, Thiele H J, Hubl W
Forschungsinstitut für Medizinische Diagnostik, Dresden, DDR.
J Clin Chem Clin Biochem. 1990 Oct;28(10):677-81.
A technique for the reproducible re-use of antibody-coated solid phases for immunoassays is described. The method based on the dissociation of the antigen-antibody complexes. Two different procedures, using glycine buffer (pH 2.3) or ethanolamine, were developed. More than ten immunoassay cycles can be realized with the same antibody-coated microtitre plates. These procedures were tested with competitive and sandwich immunoassays, monoclonal and polyclonal antibodies, and a commercial immunoassay kit.
本文描述了一种用于免疫测定的可重复使用抗体包被固相的技术。该方法基于抗原-抗体复合物的解离。开发了两种不同的程序，分别使用甘氨酸缓冲液（pH 2.3）或乙醇胺。同一抗体包被的微量滴定板可实现十多个免疫测定循环。这些程序通过竞争和夹心免疫测定、单克隆和多克隆抗体以及商用免疫测定试剂盒进行了测试。
