Misko I S, Schmidt C, Moss D J, Burrows S R, Sculley T B
Queensland Institute of Medical Research, Herston, Brisbane, Australia.
J Gen Virol. 1991 Feb;72 ( Pt 2):405-9. doi: 10.1099/0022-1317-72-2-405.
An immunodominant Epstein-Barr virus (EBV)-specific cytotoxic T lymphocyte (CTL) epitope, represented by peptide 68, has been mapped to the EBV nuclear antigen, EBNA 3. The epitope is recognized by class I-restricted CTLs through HLA-B8 and is functionally active on type A but not type B lymphoblastoid cell lines (LCLs). Herein we show that peptide 68 is not expressed as a functional CTL epitope by type A LCLs infected with an EBV B95-8 isolate. CTLs from cultures stimulated with autologous type A IARC-BL74 or QIMR-WIL LCLs lysed autologous cells stimulated with phytohaemagglutinin (PHA blasts) and coated with exogenous peptide 68. No peptide 68-specific CTLs were generated in cultures stimulated with autologous type A B95-8 or type B AG876 LCLs. However, the B95-8 LCL coated with peptide 68 was effective in the induction of a peptide-specific CTL response. A peptide 68-specific CTL clone failed to lyse the B95-8 LCL, type B AG876 LCL and PHA blasts, although such targets were lysed when coated with peptide 68.
一种以肽68为代表的免疫显性爱泼斯坦-巴尔病毒（EBV）特异性细胞毒性T淋巴细胞（CTL）表位，已被定位到EBV核抗原EBNA 3上。该表位可被通过HLA - B8的I类限制性CTL识别，并且在A型而非B型淋巴母细胞系（LCL）上具有功能活性。在此我们表明，感染了EBV B95 - 8分离株的A型LCL不会将肽68表达为功能性CTL表位。用自体A型IARC - BL74或QIMR - WIL LCL刺激培养的CTL，可裂解用植物血凝素（PHA母细胞）刺激并包被有外源性肽68的自体细胞。在用自体A型B95 - 8或B型AG876 LCL刺激的培养物中未产生肽68特异性CTL。然而，包被有肽68的B95 - 8 LCL在诱导肽特异性CTL反应方面是有效的。一个肽68特异性CTL克隆未能裂解B95 - 8 LCL、B型AG876 LCL和PHA母细胞，尽管当这些靶细胞包被有肽68时可被裂解。
