Bulovskaia L N, Borisenko G N, Drobachenko O A, Kurbatova G P, Ivanova V V
Lab Delo. 1990(10):28-30.
The authors suggest a simple easily reproducible micromethod for determination of genetically determined activity of N-acetyltransferase (EC 2.3.1.5) and acetylator phenotype. The method consists in oral intake of sulfadimezine as an acetylation substrate, followed by measurement of the enzyme activity from the degree of sulfadimezine acetylation in blood or urine samples. Examinations of 121 children with acute respiratory viral infections (ARVI) and 36 healthy children have shown that children with a slow acetylation type are more liable to ARVI. This was confirmed by the fact that complicated ARVI forms in young children occurred as a rule only in 'slow acetylators', mostly in children with the lowest N-acetyltransferase activity.
作者提出了一种简单且易于重复的微量方法，用于测定由基因决定的N - 乙酰转移酶（EC 2.3.1.5）活性及乙酰化表型。该方法包括口服磺胺二甲嘧啶作为乙酰化底物，然后根据血液或尿液样本中磺胺二甲嘧啶的乙酰化程度来测量酶活性。对121名急性呼吸道病毒感染（ARVI）儿童和36名健康儿童的检查表明，乙酰化类型为慢型的儿童更易患ARVI。这一点得到了以下事实的证实：幼儿中复杂的ARVI形式通常仅发生在“慢乙酰化者”中，大多是N - 乙酰转移酶活性最低的儿童。
