抗机械生长因子单克隆抗体

Monoclonal antibodies to mechano-growth factor.

作者信息

Kravchenko Irina V, Furalyov Vladimir A, Khotchenkov Viacheslav P, Popov Vladimir O

机构信息

A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.

出版信息

Hybridoma (Larchmt). 2006 Oct;25(5):300-5. doi: 10.1089/hyb.2006.25.300.

DOI:10.1089/hyb.2006.25.300

PMID:17044786

Abstract

Two hybridoma clones secreting monoclonal antibodies (MAbs) to mechano-growth factor (MGF) have been produced by cell fusion technique. Isotyping of the MAbs revealed that both belong to the G1 subclass. The epitope specificity of the MAbs has been examined in competition experiments. No competition was detected, suggesting that the MAbs obtained recognize different antigenic determinants. MAbs of one clone (8B9) recognize human MGF peptide absent in insulin-like growth factor-1 (IGF-1) and comprising amino acids from 87 to 111. Affinity binding constants with the full-length MGF and 87-111 amino acid peptide have been determined by enzyme-linked immunosorbent assay (ELISA). A pair of monoclonal antibodies obtained can be used in a sandwich-type assay to quantify MGF.

摘要

通过细胞融合技术制备了两个分泌针对机械生长因子（MGF）的单克隆抗体（MAb）的杂交瘤克隆。对这些单克隆抗体进行的亚型鉴定表明，它们都属于G1亚类。通过竞争实验检测了单克隆抗体的表位特异性。未检测到竞争，这表明所获得的单克隆抗体识别不同的抗原决定簇。一个克隆（8B9）的单克隆抗体识别胰岛素样生长因子-1（IGF-1）中不存在的人MGF肽，该肽由87至111位氨基酸组成。通过酶联免疫吸附测定（ELISA）确定了与全长MGF和87-111氨基酸肽的亲和结合常数。所获得的一对单克隆抗体可用于夹心型测定以定量MGF。