Su Ting, Cui Yuanbo, Zhang Xiaoai, Liu Xiaolong, Yue Jiachang, Liu Ning, Jiang Peidong
National Laboratory of Biomacromolecules, Institute of Biophysics, CAS, 15 Datun Road, Chaoyang District, Beijing 100101, PR China.
Biochem Biophys Res Commun. 2006 Dec 1;350(4):1013-8. doi: 10.1016/j.bbrc.2006.09.152. Epub 2006 Oct 6.
A Nanodevice was constructed by delta-free F(o)F(1)-ATPase within chromatophores and actin filaments through biotinlipid-streptavidin-biotin-(AC(5))(2)Sulfo-OSu system. One actin filament linking with many chromatophores functions as the Nanodevice body and many delta-free F(o)F(1)-ATPase as the Nanodevice motors. Movement of the Nanodevice was observed directly by fluorescence microscopy with CCD camera after illumination. The moving speed was about 2.17-24.43mum/s for various length Nanodevices and most of them were stopped by adding CCCP. This means that the Nanodevice was driven by PMF (proton-motive force) in the cooperating delta-free F(o)F(1)-ATPase. From bioengineering point of view, the cooperation of F(o)F(1)-ATPase is a very important research field in the future.
通过生物素脂质-链霉亲和素-生物素-(AC(5))(2)磺基琥珀酰亚胺系统，在色素体和肌动蛋白丝内构建了一种无δ F(o)F(1)-ATP酶纳米装置。一条连接多个色素体的肌动蛋白丝充当纳米装置主体，多个无δ F(o)F(1)-ATP酶充当纳米装置的马达。光照后，用带电荷耦合器件（CCD）相机的荧光显微镜直接观察纳米装置的运动。不同长度的纳米装置移动速度约为2.17-24.43μm/s，并且大多数在添加羰基氰化物间氯苯腙（CCCP）后停止运动。这意味着纳米装置是由无δ F(o)F(1)-ATP酶协同作用下的质子动力（PMF）驱动的。从生物工程学角度来看，F(o)F(1)-ATP酶的协同作用是未来一个非常重要的研究领域。
